RT Journal Article
T1 Stallion sperm selection prior to freezing using a modified colloid swim-up procedure without centrifugation
A1 Hidalgo Prieto, Manuel
A1 Ortiz Jaraba, Isabel
A1 Dorado Martín, Jesús
A1 Morrel, Jane M
A1 Gosálvez Berenguer, Jaime
A1 Consuegra González, Cesar
A1 Diaz Jimenez, María
A1 Pereira Aguilar, Blasa
A1 Crespo Castejón, Francisco
AB The aims of this study were to: 1) develop a new method for stallion sperm selection using a modified swim-up procedure through a colloid and 2) evaluate its impact in good quality eja-culates from bad freezers in comparison to methods involving centrifugation such as single layer centrifugation and sperm washing. Ejaculates were processed before freezing using three dif-ferent procedures: sperm washing (SW), colloid single layer centrifugation (SLC) and a modified colloid swim-up (SU). After semen processing, sperm recovery rates were measured and sperm were frozen. Post-thaw sperm motility (assessed by computer-assisted sperm analysis), normal forms and plasma membrane integrity (evaluated under bright-field and fluorescence microscopy respectively), and DNA fragmentation (assessed by the Sperm-Halomax kit) were compared be-tween treatments. Sperm recovery rates were similar between SU and SLC but lower than SW. Sperm motility after thawing was lower in SU in comparison to SLC and SW, maybe due to the incomplete removal of seminal plasma before freezing. Sperm DNA fragmentation was lower in SU and SLC selection methods, particularly in SLC selected samples during the first 6 h of in-cubation. The remaining sperm parameters assessed were similar among treatments. In conclu-sion, SLC is more suitable than SW and SU to process stallion semen prior to freezing, in parti-cular when sperm DNA damage is suspected. Further studies are needed in order to determine the potential benefits of SU in samples where centrifugation is not necessary, such as epididymal sperm, ejaculate fractioning or post-thaw semen samples.
PB Elsevier
SN 0378-4320
YR 2017
FD 2017-10
LK https://hdl.handle.net/20.500.14352/97292
UL https://hdl.handle.net/20.500.14352/97292
LA eng
NO 1)	Hidalgo M, Ortiz I, Dorado J, Morrell JM, Gosálvez J, Consuegra C, Diaz-Jimenez M, Pereira B, Crespo F. Stallion sperm selection prior to freezing using a modified colloid swim-up procedure without centrifugation. Anim Reprod Sci. 2017 Oct;185:83-88
NO Dr JMM provided the Androcoll-E and Halotech DNA SL the Sperm-Halomax kits used in the experiments
DS Docta Complutense
RD 17 ago 2024