RT Journal Article
T1 Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota
A1 Miguela Villoldo, Pedro
A1 Moreno, Miguel A.
A1 Rebollada Merino, Agustín Miguel
A1 Rodríguez Bertos, Antonio Manuel
A1 Hernández, Marta
A1 Rodríguez Lázaro, David
A1 Gallardo, Alejandro
A1 Quesada, Alberto
A1 Goyache Goñi, Joaquín
A1 Ugarte Ruiz, María
A1 Domínguez Rodríguez, Lucas José
AB Colistin has a long story of safe use in animals for the treatment and prevention of certain bacterial diseases. Nevertheless, the first description of the mcr-1 gene showed that colistin resistance can spread by horizontal gene transfer and changed the landscape. This study aimed to assess the effect of colistin administration on the dispersion of resistance in the microbiota of day-old broiler chicks and how the presence of mcr-1 genes influences the spread of colistin resistance determinants. In this study, 100 one-day-old chicks were divided into four groups of 25 animals (G1, G2, G3, and G4). Animals from G3/G4 were challenged with mcr-1-carrying Salmonella (day 7), while colistin (600 mg/L) was administered daily to G2/G4 animals through drinking water (from day 8 to day 15). Two quantitative PCR assays were performed to compare the amount of Salmonella and mcr-1 that were present in the caecal samples. We observed that levels of mcr-1 were higher in G3/G4 animals, especially G4, due to the spread of mcr-1-carrying Salmonella. On day 21, Salmonella levels decreased in G4, reaching similar values as those for G3, but mcr-1 levels remained significantly higher, suggesting that colistin may accelerate the spreading process when mcr-1-carrying bacteria reach the gut.
PB MPDI
SN 2079-6382
YR 2021
FD 2021-06-05
LK https://hdl.handle.net/20.500.14352/7125
UL https://hdl.handle.net/20.500.14352/7125
LA eng
NO Ministerio de Economía, Industria y Competitividad (MINECO)
NO Ministerio de Agricultura,Pesca y Alimentación/Comunidad de Madridd
NO Junta de Extremadura/FEDER
DS Docta Complutense
RD 6 abr 2025