%0 Journal Article
%A Gómez, Nestor
%A Wigdorovitz, Andres
%A Castañón, Sonia
%A Gil Dones, Félix
%A Ordá, Ricardo
%A Borca, Manuel
%A Escribano, José
%T Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
%D 2000
%@ 0304-8608
%U https://hdl.handle.net/20.500.14352/94970
%X Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major antigenic sites of the protein. Extracts from potato tubers expressing N-gS were inoculated intraperitoneally to mice, and the vaccinated mice developed serum IgG specific for TGEV. Furthermore, when potato tubers expressing N-gS were fed directly to mice, they developed serum antibodies specific for gS protein, demonstrating the oral immunogenicity of the plant derived spike protein from TGEV.
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