RT Journal Article T1 Critical attributes of formulation and of elaboration process of PLGA-protein microparticles A1 Martín Sabroso, Cristina A1 Fraguas Sánchez, Ana Isabel A1 Aparicio Blanco, Juan A1 Cano-Abad, M.F. A1 Torres Suárez, Ana Isabel AB Low drug loading, burst effect during release and drug inactivation account for the main drawbacks of protein microencapsulation in poly(d,l-lactic-co-glycolic) acid (PLGA) matrix by the water-in oil-in water (W/O/W) solvent evaporation method. Thus, the current study was set to invest the critical attributes of formulation and of elaboration process which determine protein loading into microparticles as well as its further release, using albumin as protein model.NaCl concentration in the external aqueous phase, poly(vinyl alcohol) (PVA) concentration and mostly viscosity of both the internal aqueous phase and the organic phase were critical attributes for improving drug loading, with polymer molecular weight and hydrophobicity likewise directly related to albumin loading. In such a way, when using 0.5% PVA as internal aqueous phase the highest albumin loading was achieved. Optimized microparticles exhibited a sustained in vitro release of albumin over 130 days. The influence of the microencapsulation process on albumin stability and biological activity was evaluated by carrying out cell proliferation assays on PC12 cells with albumin released from microparticles. Such assay demonstrated that the microencapsulation procedure optimized in this study did not affect the biological stability of the microencapsulated protein. SN 0378-5173 YR 2015 FD 2015-01-08 LK https://hdl.handle.net/20.500.14352/92614 UL https://hdl.handle.net/20.500.14352/92614 LA eng NO Martín-Sabroso C, Fraguas-Sánchez AI, Aparicio-Blanco J, Cano-Abad MF, Torres-Suárez AI. Critical attributes of formulation and of elaboration process of PLGA-protein microparticles. Int J Pharm 2015;480(1):27-36. NO Counseling of Education of the Community of Madrid NO European Social Fund NO Banco Santander NO Universidad Complutense de Madrid DS Docta Complutense RD 6 abr 2025