RT Journal Article
T1 Critical attributes of formulation and of elaboration process of PLGA-protein microparticles
A1 Martín Sabroso, Cristina
A1 Fraguas Sánchez, Ana Isabel
A1 Aparicio Blanco, Juan
A1 Cano-Abad, M.F.
A1 Torres Suárez, Ana Isabel
AB Low drug loading, burst effect during release and drug inactivation account for the main drawbacks of protein microencapsulation in poly(d,l-lactic-co-glycolic) acid (PLGA) matrix by the water-in oil-in water (W/O/W) solvent evaporation method. Thus, the current study was set to invest the critical attributes of formulation and of elaboration process which determine protein loading into microparticles as well as its further release, using albumin as protein model.NaCl concentration in the external aqueous phase, poly(vinyl alcohol) (PVA) concentration and mostly viscosity of both the internal aqueous phase and the organic phase were critical attributes for improving drug loading, with polymer molecular weight and hydrophobicity likewise directly related to albumin loading. In such a way, when using 0.5% PVA as internal aqueous phase the highest albumin loading was achieved. Optimized microparticles exhibited a sustained in vitro release of albumin over 130 days. The influence of the microencapsulation process on albumin stability and biological activity was evaluated by carrying out cell proliferation assays on PC12 cells with albumin released from microparticles. Such assay demonstrated that the microencapsulation procedure optimized in this study did not affect the biological stability of the microencapsulated protein.
SN 0378-5173
YR 2015
FD 2015-01-08
LK https://hdl.handle.net/20.500.14352/92614
UL https://hdl.handle.net/20.500.14352/92614
LA eng
NO Martín-Sabroso C, Fraguas-Sánchez AI, Aparicio-Blanco J, Cano-Abad MF, Torres-Suárez AI. Critical attributes of formulation and of elaboration process of PLGA-protein microparticles. Int J Pharm 2015;480(1):27-36.
NO Counseling of Education of the Community of Madrid
NO European Social Fund
NO Banco Santander
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 28 abr 2025