RT Journal Article
T1 Mycotoxins modify the barrier function of Caco-2 cells through differential gene expression of specific claudin isoforms: Protective effect of illite mineral clay
A1 Romero Martínez, Manuel Alejandro
A1 Ares Lombán, Irma
A1 Ramos Alonso, Eva
A1 Castellano Santos, Víctor Jesús
A1 Martínez Caballero, Marta
A1 Martínez Larrañaga, María Rosa
A1 Anadón Navarro, Arturo Ramón
A1 Martínez Caballero, María Aranzazu
AB Aflatoxin B1 (AFB1), fumonisin B1 (FB1), ochratoxin A (OTA) and T-2 toxin (T2) are mycotoxins that commonly contaminate the food chain and cause various toxicological effects. Their global occurrence is regarded as an important risk factor for human and animal health. In this study, the results demonstrate that, in human Caco-2 cells, AFB1, FB1, OTA and T2 origin cytotoxic effects, determining cell viability through MTT assay and LDH leakage, and decrease trans-epithelial electrical resistance (TEER). The decrease in barrier properties is concomitant with a reduction in the expression levels of the tight junction constituents claudin-3, claudin-4 and occludin. The protective effect of mineral clays (diosmectite, montmorillonite and illite) on alterations in cell viability and epithelial barrier function induced by the mycotoxins was also evaluated. Illite was the best clay to prevent the mycotoxin effects. Illite plus mycotoxin co-treatment completely abolished AFB1 and FB1-induced cytotoxicity. Also, the decreases in the gene expression of claudins and the reduction of TEER induced by mycotoxins were reversed by the illite plus mycotoxin co-treatment. In conclusion, these results demonstrated that mycotoxins AFB1, FB1, T2 and OTA disrupt the intestinal barrier permeability by a mechanism involving reduction of claudin isoform expressions, and illite counteracts this disruption.
PB Elsevier
SN 0300-483X
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/98045
UL https://hdl.handle.net/20.500.14352/98045
LA eng
NO Romero A, Ares I, Ramos E, Castellano V, Martínez M, Martínez-Larrañaga MR, Anadón A, Martínez MA. Mycotoxins modify the barrier function of Caco-2 cells through differential gene expression of specific claudin isoforms: Protective effect of illite mineral clay. Toxicology. 2016 Apr 15;353-354:21-33. doi: 10.1016/j.tox.2016.05.003. Epub 2016 May 3. PMID: 27153755.
NO Universidad Complutense de Madrid
NO Comunidad de Madrid
DS Docta Complutense
RD 3 abr 2025