RT Journal Article
T1 Preparation and characterization of full-spectrum cannabis extract loaded poly(thioether-ester) nanoparticles: In vitro evaluation of their antitumoral efficacy
A1 Freire, Nathália Freitas
A1 Feuser, Paulo Emílio
A1 Ambel, Elena Maria Tovar
A1 Cordani, Marco
A1 Pieri, Ellen De
A1 Machado de Ávila, Ricardo Andrez
A1 Zielinski, Acácio A.F.
A1 Sayer, Claudia
A1 de Araújo, Pedro Henrique Hermes
A1 Díez, Guillermo Velasco
A1 Albuquerque, Elaine Cabral
A1 Fialho, Rosana Lopes Lima
AB Δ9 -tetrahydrocannabinol (THC), cannabidiol (CBD) and other natural compounds produced by Cannabis sativa exhibit a wide array of therapeutic effects on the human body. As a result, extracts containing controlled amounts of different cannabinoids, called full spectrum extracts, have generated great interest and are currently being assayed for the management of many diseases including cancer. However, cannabinoids exhibit limited bioavailability due to their low solubility in water and moderate stability. Therefore, developing novel methods of cannabinoid administration or encapsulation that could help to improve the efficacy of treatments based on the use of these compounds is an issue of great interest. The purpose of this study was to develop biobased poly (thioether-ester)-PTEe nanoparticles containing full-spectrum Cannabis extract-CN and assay their potential efficacy in vitro cancer models. To do this we used two different approaches: 1) in-situ thiol-ene miniemulsion polymerization (Me-PTEe) and 2) thiol-ene miniemulsification/solvent evaporation method using PTEe synthesized previously by thiol-ene bulk polymerization (Se-PTEe). In both cases an α,ω-diene-diester monomer assembled from derivatives of castor oil was used. We found that CN-PTEe nanoparticles presented a high encapsulation efficiency with an average diameter of between 91 and 229 nm. Likewise, CN-PTEe nanoparticles reduced the viability to a similar extent as free CN of the cancer cell lines (B16F10, T98, and U87) but not of the non-tumoral NIH3T3 cells. Furthermore, treatment with CN-PTEe nanoparticles mimicked the working mechanism of non-encapsulated cannabinoids (inhibited the AKT signaling pathway and induced autophagy) in BF16F10 melanoma cells. These observations support the idea that the PTEe nanoparticles are effective CN nanocarriers and that they could be assayed in future studies to investigate their potential anticancer activity.
PB Elsevier
SN 0927-7757
YR 2022
FD 2022-11-25
LK https://hdl.handle.net/20.500.14352/72756
UL https://hdl.handle.net/20.500.14352/72756
LA eng
NO Ministerio de Universidades/Universidad Complutense de Madrid
NO The Recovery, Transformation and Resilience Plan "Next generation EU”
NO Instituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional(FEDER)
NO Gobierno Regional de Madrid/Fondo Europeo de Desarrollo Regional (FEDER)
NO Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - (CAPES; Brazil) - Finance Code 001
NO Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB)
NO Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; Brazil)
DS Docta Complutense
RD 3 may 2024