RT Journal Article
T1 Dynamics of Pseudomonas putida biofilms in an upscale experimental framework
A1 Espeso, David R.
A1 Martínez García, Esteban
A1 Carpio Rodríguez, Ana María
A1 De Lorenzo, Víctor
AB Exploitation of biofilms for industrial processes requires them to adopt suitable physical structures for rendering them efficient and predictable. While hydrodynamics could be used to control material features of biofilms of the platform strain Pseudomonas putida KT2440 there is a dearth of experimental data on surface-associated growth behavior in such settings. Millimeter scale biofilm patterns formed by its parental strain P. putida mt-2 under different Reynolds numbers (Re) within laminar regime were analyzed using an upscale experimental continuous cultivation assembly. A tile-scan image acquisition process combined with a customized image analysis revealed patterns of dense heterogeneous structures at Re = 1000, but mostly flattened coverings sparsely patched for Re < 400. These results not only fix the somewhat narrow hydrodynamic regime under which P. putida cells form stable coatings on surfaces destined for large-scale processes, but also provide useful sets of parameters for engineering catalytic biofilms based on this important bacterium as a cell factory.
PB Oxford University Press
SN 1367-5435
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/94055
UL https://hdl.handle.net/20.500.14352/94055
LA eng
NO Espeso, D. R., Martínez García, E., Carpio Rodríguez, A. M., Lorenzo, V. et al. «Dynamics of Pseudomonas Putida Biofilms in an Upscale Experimental Framework». Journal of Industrial Microbiology and Biotechnology, vol. 45, n.o 10, octubre de 2018, pp. 899-911.  https://doi.org/10.1007/s10295-018-2070-0.
NO Comunidad de Madrid
NO European Commission
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 14 dic 2025