RT Journal Article
T1 Sperm freezability is neither associated with the expression of aquaporin 3 nor sperm head dimensions in dromedary camel (Camelus dromedarius)
A1 O'Brien, Emma
A1 Malo, Clara
A1 Castaño, Cristina
A1 García-Casado, Pedro
A1 Toledano-Díaz, Adolfo
A1 Martínez Madrid, Carmen Belén
A1 Rodriguez-Martinez, Heriberto
A1 Álvarez-Rodríguez, Manuel
A1 Santiago-Moreno, Julián
AB The expression of aquaglyceroporin 3 (AQP-3) has been demonstrated in the spermatozoa of several mammalian species and its role has been associated with cryotolerance. Post-thaw sperm quality from individual dromedary males with different response to freezing-thawing process was evaluated through sperm head morphometry. In order to understand the cellular mechanisms affected by cryoinjury we have explored the presence and distribution of sperm AQP-3 using western blotting and immunocytochemistry. WB showed different intensity of the specific signal bands at 28 kDa. Immunofluorescence assessments allowed us to identify five different and clear AQP-3 distribution patterns of labelling in the sperm plasma membrane; acrosome, post-acrosome, mid-piece, and principal and final tail. Although expression of AQP-3 varied among male ejaculates, the individual sperm response to freeze-thawing was not associated with AQP-3 expression. Thus, AQP3 expressions do not seem like a reliable predictor of sperm response to freeze-thawing process in this species. This work is the first to describe the morphometric characteristics of the heads of dromedary spermatozoa. No correlation was found between sperm head dimensions and sperm quality variables after freeze-thawing suggesting that dromedary camel sperm head morphometry is also not a reliable predictor of cryosurvival.
PB Elsevier
SN 0093-691X
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/93908
UL https://hdl.handle.net/20.500.14352/93908
LA eng
NO O'Brien E, Malo C, Castaño C, García-Casado P, Toledano-Díaz A, Martínez-Madrid B, Rodriguez-Martinez H, Álvarez-Rodríguez M, Santiago-Moreno J. Sperm freezability is neither associated with the expression of aquaporin 3 nor sperm head dimensions in dromedary camel (Camelus dromedarius). Theriogenology. 2022 Sep 1;189:230-236
NO Este trabajo también fue financiado por un contrato entre Zoitechlab S.L. (Arquimea Group) y el INIA (CON18-141)
NO Ministerio de Ciencia e Innovación (España) 
NO European Commission 
NO Zoitechlab S.L.
NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
DS Docta Complutense
RD 12 abr 2025