RT Journal Article
T1 Electrochemical immunosensor for the determination of the cytokine interferon gamma (IFN-γ) in saliva
A1 Sánchez Tirado, Esther
A1 González Cortés, Araceli
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB A simple, fast and sensitive amperometric immunosensing method for the determination of the clinically relevant cytokine interferon gamma (IFN-γ) in saliva complying the requirements demanded for this kind of sample is reported. The target analyte was sandwiched between a specific capture antibody covalently immobilized on a screen-printed electrode functionalized by the diazonium salt grafting of p-aminobenzoic acid, and a biotinylated detector antibody labeled with a streptavidin-horseradish peroxidase conjugate. The amperometric responses measured at - 0.20 V vs Ag pseudo-reference electrode upon addition of hydrogen peroxide in the presence of hydroquinone as the redox mediator allowed a calibration plot with a linear range between 2.5 and 2000 pg mL−1 and a low limit of detection (1.6 pg mL−1) to be obtained. In addition, a good selectivity against other non-target proteins was achieved. The developed method was validated by analyzing a WHO 1st International Standard for IFN-γ. In addition, the immunosensor was used for the determination of the endogenous IFN-γ in saliva with results in excellent agreement with those obtained by a commercial ELISA kit.
PB Elsevier
SN 0039-9140
YR 2020
FD 2020-01-17
LK https://hdl.handle.net/20.500.14352/112703
UL https://hdl.handle.net/20.500.14352/112703
LA eng
NO Sánchez-Tirado, E.; González-Cortés, A.; Yáñez-Sedeño, P.; Pingarrón, J. M. Electrochemical immunosensor for the determination of the cytokine interferon gamma (IFN-γ) in saliva. Talanta 2020, 211, 120761 DOI:10.1016/j.talanta.2020.120761
DS Docta Complutense
RD 21 abr 2025