RT Journal Article
T1 Aligned copper nanowires as a cut-and-paste exclusive electrochemical transducer for free-enzyme highly selective quantification of intracellular hydrogen peroxide in cisplatin-treated cells
A1 García-Carmona, Laura
A1 Moreno Guzmán, María
A1 Martín, Aida
A1 Benito Martínez, Selma
A1 Fernández-Martínez, Ana B.
A1 González, María Cristina
A1 Lucio-Cazaña, Javier
A1 Escarpa, Alberto
AB The role and reliable quantification of intracellular hydrogen peroxide during cancer therapy constitutes an unexplored and fascinating application. In this work, we report the fabrication of vertically aligned copper nanowires (v-CuNWs) using electrosynthesis on templates, and their application as a cut-and-paste exclusive and flexible electrochemical transducer. This easily adaptable electrodic platform is demonstrated for a fast, simple and free-enzyme selective quantification of intracellular hydrogen peroxide in Cisplatin-treated human renal HK-2 cells. The v-CuNWs sensor was compared with an HRP-enzyme-based biosensor showing excellent correlation and indicates the good selectivity and analytical performance of the v-CuNWs. This sensing approach opens novel avenues for monitoring cell death processes and shows the potential of H2O2 as a cellular damage biomarker, with a clear potency for further developments for in vitro diagnosis and its implication in cancer therapy.
PB Elsevier
SN 0956-5663
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/93062
UL https://hdl.handle.net/20.500.14352/93062
LA eng
NO García-Carmona L, Moreno-Guzmán M, Martín A, Benito Martínez S, Fernández-Martínez AB, González MC, et al. Aligned copper nanowires as a cut-and-paste exclusive electrochemical transducer for free-enzyme highly selective quantification of intracellular hydrogen peroxide in cisplatin-treated cells. Biosensors and Bioelectronics 2017;96:146–51. https://doi.org/10.1016/j.bios.2017.04.048.
NO NANOAVANSENS program
NO Comunidad de Madrid
NO Ministerio de Economía y Competitividad (España)
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 13 jul 2025