RT Journal Article
T1 Sticholysin recognition of ceramide-phosphoethanolamine
A1 García Montoya, Carmen
A1 Heras Márquez, Diego
A1 Amigot Sánchez, Rafael
A1 García Linares, Sara
A1 Martínez Del Pozo, Álvaro
A1 Palacios Ortega, Juan
AB Actinoporins are pore-forming toxins produced by sea anemones. They exert their activity by binding to the membranes of target cells. There, they oligomerize, forming cation-selective pores, and inducing cell death by osmotic shock. In the early days of the field, it was shown that accessible sphingomyelin (SM) in the bilayer is required for the activity of actinoporins. While these toxins can also act on membranes composed solely of phosphatidylcholine (PC) with a high amount of cholesterol (Chol), consensus is that SM acts as a lipid receptor for actinoporins. It has been shown that the 2NH and 3OH moieties of SM are essential for actinoporin recognition. Hence, we wondered if ceramide-phosphoethanolamine (CPE) could also be recognized. Like SM, CPE has the 2NH and 3OH groups, and a positively charged headgroup. While actinoporins have been observed to affect membranes containing CPE, Chol was always also present, with the recognition of CPE remaining unclear. To test this possibility, we used sticholysins, produced by the Caribbean Sea anemone Stichodactyla helianthus. Our results show that sticholysins can induce calcein release on vesicles composed only of PC and CPE, in absence of Chol, in a way that is comparable to that induced on PC:SM membranes.
PB Elsevier
YR 2023
FD 2023-05-18
LK https://hdl.handle.net/20.500.14352/112285
UL https://hdl.handle.net/20.500.14352/112285
LA eng
NO armen García-Montoya, Diego Heras-Márquez, Rafael Amigot-Sánchez, Sara García-Linares, Álvaro Martínez-del-Pozo, Juan Palacios-Ortega, Sticholysin recognition of ceramide-phosphoethanolamine, Archives of Biochemistry and Biophysics, Volume 742, 2023, 109623, ISSN 0003-9861, https://doi.org/10.1016/j.abb.2023.109623. (https://www.sciencedirect.com/science/article/pii/S0003986123001224)
NO Banco Santander
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 31 mar 2026