RT Journal Article
T1 Time course of bilateral microglial activation in a mouse model of laser-induced glaucoma
A1 Ramírez Sebastián, Ana Isabel
A1 Hoz Montañana, María Rosa De
A1 Fernández Albarral, José
A1 García Martín, Elena Salobrar
A1 Rojas López, María Blanca
A1 Valiente Soriano, Francisco J.
A1 Avilés Trigueros, Marcelino
A1 Villegas Pérez, María P.
A1 Vidal Sanz, Manuel
A1 Triviño Casado, Alberto
A1 Ramírez Sebastián, José Manuel
A1 Salazar Corral, Juan José
AB Microglial activation is associated with glaucoma. In the model of unilateral laser-induced ocular hypertension (OHT), the time point at which the inflammatory process peaks remains unknown. Different time points (1, 3, 5, 8, and 15 d) were compared to analyze signs of microglial activation both in OHT and contralateral eyes. In both eyes, microglial activation was detected in all retinal layers at all time points analyzed, including: i) increase in the cell number in the outer segment photoreceptor layer and plexiform layers (only in OHT eyes) from 3 d onward; ii) increase in soma size from 1 d onward; iii) retraction of the processes from 1 d in OHT eyes and 3 d in contralateral eyes; iv) increase in the area of the retina occupied by Iba-1+ cells in the nerve fiber layer/ganglion cell layer from 1 d onward; v) increase in the number of vertical processes from 1 d in contralateral eyes and 3 d in OHT eyes. In OHT eyes at 24 h and 15 d, most Iba-1+ cells were P2RY12+ and were down-regulated at 3 and 5 d. In both eyes, microglial activation was stronger at 3 and 5 d (inflammation peaked in this model). These time points could be useful to identify factors implicated in the inflammatory process.
PB Nature Research
SN 2045-2322
YR 2020
FD 2020-03-17
LK https://hdl.handle.net/20.500.14352/103770
UL https://hdl.handle.net/20.500.14352/103770
LA eng
NO Ramírez, A.I., de Hoz, R., Fernández-Albarral, J.A. et al. Time course of bilateral microglial activation in a mouse model of laser-induced glaucoma. Sci Rep 10, 4890 (2020). https://doi.org/10.1038/s41598-020-61848-9
NO Received: 24 October 2018; Accepted: 03 March 2020; Published: 17 March 2020
NO Ministerio de Economía y Competitividad (España)
NO Ministerio de Ciencia e Inovación (España)
NO Ministerio de Educación, Cultura y Deporte (España)
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 17 ago 2024