RT Journal Article
T1 Unlocking the potential of NH2-MIL-101 (Fe) nanoMOF for advanced electrochemical immunosensing in chronic wound diagnostics
A1 Pérez Ginés, Víctor
A1 Valero-Calvo, David
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Pedrero Muñoz, María
A1 García Alonso, Francisco J.
A1 Pingarrón Carrazón, José Manuel
A1 Campuzano Ruiz, Susana
A1 Escosura Muniz, Alfredo de la
AB In this work we take advantage, for the first time, of the porous and functional structure of a nanoscale metal organic framework (nanoMOF), specifically NH2-MIL-101 (Fe) nanoMOF, for its use as advantageous enzyme-carrier nanotag in immunoassays. In particular, the porous structure of the nanoMOF allows accommodating a high loading of enzyme molecules, which, in addition, are protected from degradation inside the pores providing a high stability. This property together with the ability of the nanoMOF to link antibodies through its functional groups make it a very robust and powerful label. Both the enhanced sensitivity, compared with the conventional use of enzyme-labelled antibodies, and the high long-term stability are demonstrated. Such nanotag also offers advantages related to ease of modification and cost effectiveness. The detection of myeloperoxidase (MPO), a key biomarker for chronic wound monitoring, is proposed to demonstrate the applicability of this approach. A sandwich-type immunoassay using magnetic beads (MBs)-based platforms and subsequent amperometric detection allows the determination of MPO in the clinical range (5 – 100 ng mL−1), with a limit of detection of 1.4 ng mL−1, and good reproducibility (RSD(n = 10) = 7.1 %). The developed bio-strategy also exhibits high selectivity against potential interferents and a good stability of the nanotag for at least 2 months. Moreover, the developed method was successfully implemented to determine MPO in simulated chronic wound matrices, demonstrating its feasibility in a real scenario.
PB Elsevier
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/123521
UL https://hdl.handle.net/20.500.14352/123521
LA eng
NO Víctor Pérez-Ginés, David Valero-Calvo, Rebeca M. Torrente-Rodríguez, María Pedrero, Francisco J. García-Alonso, José M. Pingarrón, Susana Campuzano, Alfredo de la Escosura-Muñiz, Unlocking the potential of NH2-MIL-101 (Fe) nanoMOF for advanced electrochemical immunosensing in chronic wound diagnostics, Sensors and Actuators B: Chemical, Volume 432, 2025, 137460, ISSN 0925-4005, https://doi.org/10.1016/j.snb.2025.137460.
NO Asturias Regional Government
NO Ministerio de Ciencia e Innovación español
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 2 sept 2025