RT Journal Article
T1 Drug-Free Enzyme-Based Bactericidal Nanomotors against Pathogenic Bacteria
A1 Vilela García, Diana
A1 Blanco Cabra, Nuria
A1 Eguskiza, Ander
A1 Hortelao, Ana C.
A1 Torrents, Eduard
A1 Sánchez, Samuel
AB The low efficacy of current conventional treatments for bacterial infections increases mortality rates worldwide. To alleviate this global health problem, we propose drug-free enzymebased nanomotors for the treatment of bacterial urinary-tract infections. We develop nanomotors consisting of mesoporous silica nanoparticles (MSNPs) that were functionalized with either urease (U-MSNPs), lysozyme (L-MSNPs), or urease and lysozyme (MMSNPs), and use them against nonpathogenic planktonic Escherichia coli. U-MSNPs exhibited the highest bactericidal activity due to biocatalysis of urea into NaHCO3 and NH3, which also propels U-MSNPs. In addition, U-MSNPs in concentrations above 200 μg/mL were capable of successfully reducing 60% of the biofilm biomass of a uropathogenic E. coli strain. This study thus provides a proof-of-concept, demonstrating that enzyme-based nanomotors are capable of fighting infectious diseases. This approach could potentially be extended to other kinds of diseases by selecting appropriate biomolecules.
PB American Chemical Society (ACS)
SN 1944-8244
YR 2021
FD 2021-03-26
LK https://hdl.handle.net/20.500.14352/6745
UL https://hdl.handle.net/20.500.14352/6745
LA eng
NO CRUE-CSIC (Acuerdos Transformativos 2021)
NO Ministerio de Economía y Competitividad (España)
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Centro de Excelencia Severo Ochoa
NO Generalitat de Catalunya
NO European Research Council
NO Unión Europea. Horizonte 2020
NO BBVA Foundation
NO La Caixa Foundation
NO Agencia Estatal de Investigación (España)
DS Docta Complutense
RD 5 abr 2025