RT Journal Article T1 Residual brewing yeast as a source of polyphenols: Extraction, identification and quantification by chromatographic and chemometric tools T2 Levadura de cerveza residual como fuente de polifenoles: extracción, identificación y cuantificación mediante métodos cromatográficos y herramientas quimiométricas A1 León González, María Eugenia De A1 Gómez Mejía, Esther A1 Rosales Conrado, Noelia A1 Madrid Albarrán, María Yolanda AB A method combining aqueous extraction, reversed-phase high-performance capillary liquid chromatography with photodiode array detection (cLC-DAD) and chemometric tools, was developed to determine phenolic compounds in residual brewing yeast. The effect of temperature, nature of extraction solvent and method for separation of extract solution were studied to optimize the extraction conditions on the basis of total phenolic content (TPC), total flavonoids content (TFC) and antioxidant capacity. Polyphenols were determined by cLC-DAD. Flavonols as rutin and kaempferol, flavonoids as naringin, phenolic acids as gallic acid and antioxidants as trans-ferulic and p-coumaric acids were found and quantified in the brewing residue. Data were subjected to evaluation using multifactor ANOVA and principal component analysis (PCA), both showing that lyophilization pretreatment affects the content of individual polyphenols and that residual brewing yeast contains higher polyphenol amounts than the liquid beer waste. The obtained results suggest that residual brewing yeast could be a source of polyphenols. PB Elsevier Sci Ltd SN 0308-8146 YR 2018 FD 2018 LK https://hdl.handle.net/20.500.14352/92816 UL https://hdl.handle.net/20.500.14352/92816 LA eng NO León-González, M.E., Gómez-Mejía, E., Rosales-Conrado, N., Madrid-Albarrán, Y., Residual brewing yeast as a source of polyphenols: extraction, identification and quantification by chromatographic and chemometric tools, Food Chemistry 267 (2018) 246-254, doi: https://doi.org/10.1016/j.foodchem.2017.06.141 NO Comunidad de Madrid NO Ministerio de Economía y Competitivad (España) NO European Commission DS Docta Complutense RD 6 oct 2024