TY  - JOUR
AU  - Rico San Román, Laura
AU  - Hänggeli, Kai Pascal Alexander
AU  - Hemphill, Andrew
AU  - Horcajo Iglesias, María Del Pilar
AU  - Collantes Fernández, Esther
AU  - Ortega Mora, Luis Miguel
AU  - Boubaker, Ghalia
PY  - 2024
DO  - 10.3389/fcimb.2024.1419209
UR  - https://hdl.handle.net/20.500.14352/107362
T2  - Frontiers in Cellular and Infection Microbiology
AB  - As for many other organisms, CRISPR-Cas9 mediated genetic modification has gained increasing importance for the identification of vaccine candidates and drug targets in Neospora caninum, an apicomplexan parasite causing abortion in cattle and...
LA  - eng
M2  - 1
PB  - Frontiers
KW  - Duplex TaqMan-qPCR
KW  - Single TaqMan-qPCR
KW  - DHFR-TS
KW  - Mdhfr-ts
KW  - CRISPR-Cas9
KW  - Off target effects
KW  - Neospora caninum
KW  - Toxoplasma gondii
TI  - TaqMan-quantitative PCR assays applied in Neospora caninum knock-outs generated through CRISPR-Cas9 allow to determine the copy numbers of integrated dihydrofolate reductase-thymidylate synthase drug selectable markers
TY  - journal article
VL  - 14
ER  -