RT Journal Article
T1 Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride
A1 Carpena Torres, Carlos
A1 Pintor, Jesús
A1 Pérez de Lara, María Jesús
A1 Huete Toral, Fernando
A1 Crooke, Almudena
A1 Pastrana, Cristina
A1 Carracedo Rodríguez, Juan Gonzalo
AB Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium  chloride (BAC), reduce the days of instillation of the original model by increasing the  concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and  randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily.  Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining,  conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of  IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for  5 consecutive days, there was a significant increase in tear secretion without anesthesia (P <  0.001), corneal staining (P < 0.001), conjunctival hyperemia (P < 0.001), and levels of IL-6 mRNA  (P = 0.005) compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia (P < 0.001), tear breakup time (P = 0.007), tear osmolarity (P < 0.001), density of  goblet cells (P < 0.001), and height of mucin cloud (P < 0.001). Conclusions. )e topical  instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a  rabbit dry eye model, reducing the number of days of instillation compared to the original model  (14 days).
PB Hindawi Publishing Corporation
SN 2090-004X
YR 2020
FD 2020-05-30
LK https://hdl.handle.net/20.500.14352/6300
UL https://hdl.handle.net/20.500.14352/6300
LA eng
NO This study was published in memory of Jesus Pintor, who passed away on 2 April 2019. This study has been self-funded.
DS Docta Complutense
RD 30 abr 2024