RT Journal Article
T1 Oral immunization with heat-inactivated Mycobacterium bovis reduces local parasite dissemination and hepatic granuloma development in mice infected with Leishmania amazonensis
A1 Ferreras Colino, Elisa
A1 Moreno, Inmaculada
A1 Gortázar, Christian
A1 Sevilla, Iker
A1 Agulló Ros, Irene
A1 Domínguez Rodríguez, Lucas José
A1 Juste, Ramón
A1 Risalde, Maria A.
A1 Domínguez, Mercedes
AB Aiming to explore whether oral immunization with heat-inactivated Mycobacterium bovis (HIMB) protects mice against Leishmania infection, 18 female BALB/c mice were randomly assigned to the immunized group, that received oral HIMB, or the control group, and were infected by inoculation of 10,000 Leishmania amazonensis promastigotes in the footpad. Spleen culture was positive in 55.55% of immunized mice and in 100% of control mice (p = 0.082). The number of immunolabeled amastigotes number in the popliteal lymph node was lower in the immunized group (p = 0.009). The immunized group presented fewer mature granulomas in the liver (p = 0.005) and more Lys + macrophages (p = 0.002) and fewer CD3+ T lymphocytes (p < 0.001) per hepatic granuloma. We conclude that immunization with HIMB via the oral route limited local parasite dissemination and hepatic granuloma development in mice challenged with Leishmania amazonensis through stimulation of macrophages, which is compatible with trained immunity.
SN 0034-5288
YR 2023
FD 2023-09
LK https://hdl.handle.net/20.500.14352/107484
UL https://hdl.handle.net/20.500.14352/107484
LA eng
NO Ferreras-Colino, E., Moreno, I., Gortázar, C., Sevilla, I., Agulló-Ros, I., Domínguez, L., Juste, R., Risalde, M. A., & Domínguez, M. (2023). Oral immunization with heat-inactivated Mycobacterium bovis reduces local parasite dissemination and hepatic granuloma development in mice infected with Leishmania amazonensis. Research in veterinary science, 162, 104963. https://doi.org/10.1016/j.rvsc.2023.104963
NO Author contributionsConcept formulation: MAR, CG, IM, MD, IS, RJ, LD. Methodology: EFC, IM, MD, IAR, MAR. Data analysis: EFC, IM, MD. Writing the original draft: EFC. Editing: MAR, CG, IM, MD, IS, RJ, LD, IAR. Funding acquisition: CG, MAR. All authors read and approved the final manuscript.
NO Junta de Castilla-La Mancha
NO Ministerio de Ciencia, Edsucación y Universidades
NO European Social Fund
NO Ministerio de Ciencia, Educación y Universidades
DS Docta Complutense
RD 5 abr 2025