RT Journal Article
T1 In vitro and in vivo production and split-intein mediated ligation (SIML) of circular bacteriocins
A1 Bland, Michael J.
A1 Sevillano, Ester
A1 Lafuente, Irene
A1 Mohamed El Bakkoury, 
A1 Philippe Gabant, 
A1 Peña Vidal, Nuria
A1 Muñoz Atienza, Estefanía
A1 Cintas Izarra, Luis Miguel
A1 Hernández Cruza, Pablo Elpidio
A1 Borrero Del Pino, Juan
AB Circular bacteriocins are antimicrobial peptides produced by bacteria that after synthesis undergo a head-to-tail circularization. Compared to their linear counterparts, circular bacteriocins are, in general, very stable to temperature and pH changes and more resistant to proteolytic enzymes, being considered as one of the most promising groups of antimicrobial peptides for their potential biotechnological applications. Up to now, only a reduced number of circular bacteriocins have been identified and fully characterized, although many operons potentially coding for new circular bacteriocins have been recently found in the genomes of different bacterial species. The production of these peptides is very complex and depends on the expression of different genes involved in their synthesis, circularization, and secretion. This complexity has greatly limited the identification and characterization of these bacteriocins, as well as their production in heterologous microbial hosts. In this work, we have evaluated a synthetic biology approach for the <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic> production combined with a split-intein mediated ligation (SIML) of the circular bacteriocin garvicin ML (GarML). The expression of one single gene is enough to produce a protein that after intein splicing, circularizes in an active peptide with the exact molecular mass and amino acid sequence as native GarML. <jats:italic>In vitro</jats:italic> production coupled with SIML has been validated with other, well described and not yet characterized, circular bacteriocins. The results obtained suggest that this synthetic biology tool holds great potential for production, engineering, improving and testing the antimicrobial activity of circular bacteriocins.
PB Frontiers
SN 1664-302X
YR 2022
FD 2022-11
LK https://hdl.handle.net/20.500.14352/108764
UL https://hdl.handle.net/20.500.14352/108764
LA eng
NO Peña, N., Bland, M. J., Sevillano, E., Muñoz-Atienza, E., Lafuente, I., Bakkoury, M. E., Cintas, L. M., Hernández, P. E., Gabant, P., & Borrero, J. (2022). In vitro and in vivo production and split-intein mediated ligation (Siml) of circular bacteriocins. Frontiers in Microbiology, 13, 1052686. https://doi.org/10.3389/fmicb.2022.1052686
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 22 ene 2026