RT Journal Article
T1 Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes
A1 Daza, Rafael
A1 Cruces González, Julia
A1 Arroyo Hernández, María
A1 Marí-Buyé, Núria
A1 Fuente del Rey, Mónica de la
A1 Plaza, Gustavo R.
A1 Elices Calafat, Manuel
A1 Pérez Rigueiro, José
A1 Guinea, Gustavo V.
AB The mechanical behavior of living murine T-lymphocytes was assessed by atomic force microscopy (AFM). A robust experimental procedure was developed to overcome some features of lymphocytes, in particular their spherical shape and non-adherent character. The procedure included the immobilization of the lymphocytes on amine-functionalized substrates, the use of hydrodynamic effects on the deflection of the AFM cantilever to monitor the approaching, and the use of the jumping mode for obtaining the images. Indentation curves were analyzed according to Hertz's model for contact mechanics. The calculated values of the elastic modulus are consistent both when considering the results obtained from a single lymphocyte and when comparing the curves recorded from cells of different specimens.
PB IOP Publishing
SN 1478-3967, ESSN: 1478-3975
YR 2015
FD 2015-03-19
LK https://hdl.handle.net/20.500.14352/23315
UL https://hdl.handle.net/20.500.14352/23315
LA eng
NO Ministerio de Educación y Ciencia
NO Comunidad de Madrid
NO Fundación Marcelino Botín
DS Docta Complutense
RD 18 abr 2025