TY - JOUR AU - Kotzamanis, George AU - Cheung, Wing AU - Abdulrazzak, Hassan AU - PĂ©rez Luz, Sara AU - Howe, Steven AU - Cooke, Howard AU - Huxley, Clare PY - 2005 DO - 10.1016/j.gene.2005.01.017 SN - 0378-1119 UR - https://hdl.handle.net/20.500.14352/93087 T2 - Gene AB - Human artificial chromosomes (HACs) can be formed de novo by transfection of large fragments of cloned alphoid DNA into human HT1080 cells in tissue culture. In order to generate HACs carrying a gene of interest, one can either co-transfect the... LA - eng M2 - 29 KW - Homologous recombination KW - HAC KW - MAC KW - HPRT KW - BAC KW - Centromere KW - Minichromosome TI - Construction of human artificial chromosome vectors by recombineering TY - journal article VL - 351 ER -