RT Journal Article
T1 Different strategies to obtain laccase-based time-temperature indicator for monitoring of cold chain storage and management
A1 Hackenhaar, Camila Regina
A1 Zimmermann, Vinicius
A1 Linares, Lucas dos Santos
A1 Cannavon, Victor Moreira
A1 Bolívar Bolívar, Juan Manuel
A1 Fernandez Lafuente, Roberto
A1 Rodrigues, Rafael C.
AB In this work, we have developed a battery of enzymatic time-temperature indicators (TTIs) based on the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) catalyzed by laccase from Trametes versicolor. For this, enzyme concentration, pH value, and addition of sodium azide (a non-competitive inhibitor of the enzyme) were utilized to modulate the apparent activation energy of the reaction catalyzed by the biocatalyst (Ea) and the reaction rate constant, tailoring them to match specific product requirements. The color change (∆E) was used as the response parameter. To check the feasibility of this biosensor, ∆E was modeled using a modified Arrhenius equation to extract kinetic parameters for each TTI formulation. TTI prototypes were evaluated under a wide range of isothermal (4–45 °C) and dynamic temperature conditions (4–30 °C). The results showed that enzyme concentration primarily influenced the (∆E) rate, while reaction Ea was significantly affected by pH and laccase inhibitor concentration. Under dynamic conditions simulating real cold chain scenarios, the model accurately predicted the TTI response, demonstrating high correlation between experimental and simulated data (R2 > 0.94). These findings confirm the feasibility of customizing TTIs for different perishable products. These enzymatic TTIs offer a scalable and cost-effective tool for integration into intelligent packaging systems, enhancing cold chain management and reducing food waste
PB Elsevier
SN 1466-8564
YR 2025
FD 2025-07-21
LK https://hdl.handle.net/20.500.14352/131800
UL https://hdl.handle.net/20.500.14352/131800
LA eng
NO Camila Regina Hackenhaar, Vinicius Zimmermann, Lucas dos Santos Linares, Victor Moreira Cannavon, Juan M. Bolivar, Roberto Fernandez-Lafuente, Rafael C. Rodrigues, Different strategies to obtain laccase-based time-temperature indicator for monitoring of cold chain storage and management, Innovative Food Science & Emerging Technologies, Volume 109, 2026, 104454, ISSN 1466-8564, https://doi.org/10.1016/j.ifset.2026.104454.
NO MICIU/AEI
NO MINECO and AEI
NO European Union NextGeneration EU/PRTR
DS Docta Complutense
RD 18 mar 2026