RT Journal Article
T1 Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection
A1 Laurenti, Marco
A1 Paez-Pérez, Miguel
A1 Algarra González, Manuel
A1 Alonso Cristobal, Paulino
A1 López Cabarcos, Enrique
A1 Méndez González, Diego
A1 Rubio Retama, Benito Jorge
AB We developed a sensor for the detection of specific microRNA (miRNA) sequences that was based on graphene quantum dots (GQDs) and ssDNA-UCNP@SiO2. The proposed sensor exploits the interaction between the sp2 carbon atoms of the GQD, mainly π–π stacking, and the DNA nucleobases anchored on the upconversion nanoparticles (UCNPs). This interaction brings the GQD to the surface of the ssDNA-UCNP@SiO2 system, enhancing the upconversion emission. On the other hand, hybridization of the single-stranded DNA (ssDNA) chains anchored on the nanoparticles with their complementary miRNA sequences blocks the capacity of the UCNPs to interact with the GQD through π–π stacking. That gives as result a reduction of the fluorescent enhancement, which is dependent on the concentration of miRNA sequences. This effect was used to create a sensor for miRNA sequences with a detection limit of 10 fM.
PB American Chemical Society 
SN 1944-8244
YR 2016
FD 2016-05-25
LK https://hdl.handle.net/20.500.14352/101129
UL https://hdl.handle.net/20.500.14352/101129
LA eng
NO Laurenti M, Paez-Perez M, Algarra M, Alonso-Cristobal P, Lopez-Cabarcos E, Mendez-Gonzalez D, et al. Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection. ACS Appl Mater Interfaces 2016;8:12644–51. https://doi.org/10.1021/acsami.6b02361.
NO Bill & Melinda Gates Foundation
NO Ministerio de Economía, Comercio y Empresa (España)
DS Docta Complutense
RD 17 jul 2024