RT Journal Article
T1 From the microbiome to the electrome: implications for the microbiota–gut–brain axis
A1 Bourqqia-Ramzi, Marwane
A1 Mansilla-Guardiola, Jesús
A1 Muñoz Rodríguez, David
A1 Quarta, Elisa
A1 Lombardo Hernandez, Juan
A1 Murciano Cespedosa, Antonio
A1 Conejero Meca, Francisco José
A1 Mateos González, Álvaro
A1 Geuna, Stefano
A1 García Esteban, María Teresa
A1 Herrera Rincón, Celia
AB The gut microbiome plays a fundamental role in metabolism, as well as the immune and nervous systems. Microbial imbalance (dysbiosis) can contribute to subsequent physical and mental pathologies. As such, interest has been growing in the microbiota–gut–brain brain axis and the bioelectrical communication that could exist between bacterial and nervous cells. The aim of this study was to investigate the bioelectrical profile (electrome) of two bacterial species characteristic of the gut microbiome: a Proteobacteria Gram-negative bacillus Escherichia coli (E. coli), and a Firmicutes Gram-positive coccus Enterococcus faecalis (E. faecalis). We analyzed both bacterial strains to (i) validate the fluorescent probe bis-(1,3-dibutylbarbituric acid) trimethine oxonol, DiBAC4(3), as a reliable reporter of the changes in membrane potential (Vmem) for both bacteria; (ii) assess the evolution of the bioelectric profile throughout the growth of both strains; (iii) investigate the effects of two neural-type stimuli on Vmem changes: the excitatory neurotransmitter glutamate (Glu) and the inhibitory neurotransmitter γ-aminobutyric acid (GABA); (iv) examine the impact of the bioelectrical changes induced by neurotransmitters on bacterial growth, viability, and cultivability using absorbance, live/dead fluorescent probes, and viable counts, respectively. Our findings reveal distinct bioelectrical profiles characteristic of each bacterial species and growth phase. Importantly, neural-type stimuli induce Vmem changes without affecting bacterial growth, viability, or cultivability, suggesting a specific bioelectrical response in bacterial cells to neurotransmitter cues. These results contribute to understanding the bacterial response to external stimuli, with potential implications for modulating bacterial bioelectricity as a novel therapeutic target.
PB MDPI
SN 1661-6596
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/112450
UL https://hdl.handle.net/20.500.14352/112450
LA eng
NO Bourqqia-Ramzi, M., Mansilla-Guardiola, J., Muñoz-Rodriguez, D., Quarta, E., Lombardo-Hernandez, J., Murciano-Cespedosa, A., Conejero-Meca, F. J., Mateos González, Á., Geuna, S., Garcia-Esteban, M. T., & Herrera-Rincon, C. (2024). From the Microbiome to the Electrome: Implications for the Microbiota–Gut–Brain Axis. International Journal of Molecular Sciences, 25(11), 6233. https://doi.org/10.3390/ijms25116233
NO This research was funded by the Ramon y Cajal program through the Spanish Ministry of Science, Research Agency (RYC2020-029499-I) and by the Computense University of Madrid (Research Project PR3/23-30827) to C.H-R.
NO Ministerio de Ciencia e Innovación (España)
NO Universidad Complutense de Madrid
NO European Commission
DS Docta Complutense
RD 6 abr 2025