RT Journal Article
T1 Magnetic microbeads-based amperometric immunoplatform for the rapid and sensitive detection of N6-methyladenosine to assist in metastatic cancer cells discrimination
A1 Povedano Muñumel, Eloy
A1 Gamella Carballo, María
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Montero-Calle, Ana
A1 Pedrero Muñoz, María
A1 Solís-Fernández, Guillermo
A1 Navarro Villoslada, Fernando
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
A1 Pingarrón Carrazón, José Manuel
AB This work describes the preparation of an immunoplatform for the sensitive and selective determination of N6-methyladenosine (m6A). The simple and fast protocol involves for the first time the use of micromagnetic immunoconjugates to establish a direct competitive assay between the m6A target and a biotinylated RNA oligomer bearing a single m6A enzymatically labelled with a commercial conjugate of streptavidin-peroxidase (Strep-HRP) as tracer. The cathodic current change measured in the presence of H2O2/hydroquinone (HQ) at screen-printed carbon electrodes (SPCEs) upon surface capturing the magnetic bioconjugates is inversely proportional to the m6A target concentration. After evaluating the effect of key variables, the analytical characteristics were established for the determination of three different targets: the N6-methyladenosine-5′ -triphosphate (m6ATP) ribonucleotide, a short synthetic RNA oligomer bearing a single m6A and the positive control provided in a commercial colorimetric kit for m6A-RNA quantification. The obtained results show that this immunoplatform is competitive with other methods reported to date, achieving an improved sensitivity (limit of detection of 0.9 pM for the short synthetic oligomer) using a much simpler and faster protocol (~1 h) and disposable electrodes for the transduction. Furthermore, the applicability for discriminating the metastatic potential of cancer cells by directly analyzing a small amount of raw total RNA without enriching or fragmenting was also preliminary assessed.
PB Elsevier
SN 0956-5663
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/91814
UL https://hdl.handle.net/20.500.14352/91814
LA eng
NO Povedano, Eloy, et al. «Magnetic Microbeads-Based Amperometric Immunoplatform for the Rapid and Sensitive Detection of N6-Methyladenosine to Assist in Metastatic Cancer Cells Discrimination». Biosensors and Bioelectronics, vol. 171, enero de 2021, p. 112708. https://doi.org/10.1016/j.bios.2020.112708.
DS Docta Complutense
RD 18 abr 2025