RT Journal Article
T1 Validation of a rapid and easy-to-apply method to simultaneously quantify co-loaded dexamethasone and melatonin plgamicrospheres by hplc-uv: encapsulation efficiency and in vitro release
A1 Vicario De La Torre, Marta
A1 Herrero Vanrell, María Del Rocío
A1 Andrés Guerrero, Vanesa
A1 Bravo Osuna, Irene
A1 Molina Martínez, Irene Teresa
A1 Brugnera, Marco
AB This paper discusses the development and validation of a rapid method for the reversed phase HPLC-UV quantification of biodegradable poly(D,L-lactic-co-glycolic) acid (PLGA) micro-spheres co-loaded with two neuroprotective agents (dexamethasone and melatonin) (DX-MEL-MSs) to be intravitreally administered as a promising glaucoma treatment. The study was performed to validate two procedures that quantify the content of the two active substances entrapped into the polymer matrix during an encapsulation efficiency assay and the amount of drugs liberated over time during the in vitro release assay. The reversed-phase method allowed for the simultaneous determination of dexamethasone and melatonin, which were respectively detected at 240.5 and 222.7 nm. Chromatographic separation was performed using an Ascentis® C18 HPLC Column (25 cm × 4.6 mm, 5 μm) with an isocratic mobile phase composed of methanol-water (70:30, v/v) with 1.0 mL min−1 flow rate. The two procedures were validated analytically in terms of system suitability testing, specificity, linearity, precision, accuracy, sensitivity, and robustness. Both the validated procedures were applied to characterize DX-MEL-MSs and were found appropriate to quantify the drug quantities encapsulated and estimate their release profile over 10 days. The validation study designed in this work can be helpful for planning any other protocols that refer to the quantification of PLGA based drug delivery systems.
PB MDPI
YR 2022
FD 2022-01-26
LK https://hdl.handle.net/20.500.14352/115544
UL https://hdl.handle.net/20.500.14352/115544
LA eng
NO Brugnera, M.; Vicario-de-la- Torre, M.; Andrés-Guerrero, V.; Bravo-Osuna, I.; Molina-Martínez, I.T.; Herrero-Vanrell, R. Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Encapsulation Efficiency and In Vitro Release. Pharmaceutics 2022, 14, 288. https://doi.org/ 10.3390/pharmaceutics14020288
NO Universidad Complutense de Madrid. Research Group UCM 920415.
NO Horizon 2020 research and innovation programme (European Commission)
DS Docta Complutense
RD 10 abr 2025