RT Journal Article
T1 Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk
A1 Roy, A.
A1 Infantes-Lorenzo, J.A.
A1 Dominguez, M.
A1 Moreno, I.
A1 Pérez Sancho, Marta
A1 García Benzaquén, Nerea
A1 García-Seco Romero, María Teresa
A1 Álvarez Sánchez, Julio
A1 Romero Martínez, Beatriz
A1 Gortázar, C.
A1 Juan Ferré, Lucía De
A1 Domínguez Rodríguez, Lucas José
A1 Bezos Garrido, Javier
AB Caprine tuberculosis (TB) is a zoonosis with sanitary and economic repercussions. Caprine TB control programs are based on a test and cull strategy using the intradermal tuberculin tests and slaughterhouse surveillance. However, this approach is not always feasible and may have a limited sensitivity under specific circumstances. In this study, performance of a new experimental test based on the P22 protein complex (P22 ELISA) was evaluated in two TB-infected herds using milk and serum samples and compared with cell-based diagnostic tests. Samples from a low (n = 62, herd 1) and a high (n = 52, herd 2) TB prevalence herd were selected. Moreover, bulk tank milk samples from both herds were analysed using the P22 ELISA. At the end of the study, a group of animals (n = 21) was euthanized and subjected to post-mortem analysis and bacteriological culture. Significant differences (p < .001) on the qualitative and quantitative (ODs) results were observed between herds using both serum and milk samples in the P22 ELISA. The correlation observed in the quantitative results  obtained in serum and milk samples was very strong in animals from flock 2 (rs = 0.91) and moderate in animals from flock 1 (rs = 0.46). Among the slaughtered animals, the P22 ELISA detected a higher proportion of lesion-culture positive animals than cell-based diagnostic tests (61.9 and 66.7% using milk and serum samples, respectively). The P22 ELISA using milk samples demonstrated a similar sensitivity compared with serum samples, suggesting it might be a valuable test for TB control in dairy goats.
PB ELSEVIER SCI LTD
SN 0034-5288
YR 2020
FD 2020-02-01
LK https://hdl.handle.net/20.500.14352/93822
UL https://hdl.handle.net/20.500.14352/93822
LA eng
NO Roy A, Infantes-Lorenzo JA, Dominguez M, Moreno I, Perez-Sancho M, Garcia N, Garcia-Seco T, Alvarez J, Romero B, Gortazar C, de Juan L, Dominguez L and Bezos J*. Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk. Research in Veterinary Science, 128:217-223. 2020. DOI: 10.1016/j.rvsc.2019.12.009
NO Financiado por proyecto RTA2015–00043-C02–02;Impact factor: 2.534. Category: Veterinary Sciences, Quartile: 1, Position: 30 of 146.
NO Ministerio de Agricultura, Pesca y Alimentación (RTA2015–00043-C02–02)
NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (RTA2015–00043-C02–02)
NO Ministerio de Economía y Competitividad
DS Docta Complutense
RD 4 ago 2024