RT Journal Article
T1 Fast and Sustained Axonal Growth by BDNF Released from Chitosan Microspheres
A1 Aranaz Corral, Inmaculada
A1 Acosta Contreras, Florentina Niuris
A1 Revuelta Crespo, Julia
A1 Bastida Codina, Agatha
A1 Gómez Casado, Víctor
A1 Civera Tejuca, María Concepción
A1 Garrido Fernández, Leoncio
A1 García Junceda, Eduardo
A1 Heras Caballero, Ángeles María
A1 Alcántara León, Andrés Rafael
A1 Fernández Mayoralas, Alfonso
A1 Doncel Pérez, Ernesto
AB Brain-derived neurotrophic factor (BDNF) regulates dendritic branching and dendritic spine morphology, as well as synaptic plasticity and long-term potentiation. Consequently, BDNF deficiency has been associated with some neurological disorders such as Alzheimer’s, Parkinson’s or Huntington’s diseases. In contrast, elevated BDNF levels correlate with recovery after traumatic central nervous system (CNS) injuries. The utility of BDNF as a therapeutic agent is limited by its short half-life in a pathological microenvironment and its low efficacy caused by unwanted consumption of non-neuronal cells or inappropriate dosing. Here, we tested the activity of chitosan microsphere-encapsulated BDNF to prevent clearance and prolong the efficacy of this neurotrophin. Neuritic growth activity of BDNF release from chitosan microspheres was observed in the PC12 rat pheochromocytoma cell line, which is dependent on neurotrophins to differentiate via the neurotrophin receptor (NTR). We obtained a rapid and sustained increase in neuritic out-growth of cells treated with BDNF-loaded chitosan microspheres over control cells (p < 0.001). The average of neuritic out-growth velocity was three times higher in the BDNF-loaded chitosan microspheres than in the free BDNF. We conclude that the slow release of BDNF from chitosan microspheres enhances signaling through NTR and promotes axonal growth in neurons, which could constitute an important therapeutic agent in neurodegenerative diseases and CNS lesions
PB MDPI
SN 1660-3397
YR 2023
FD 2023-01-27
LK https://hdl.handle.net/20.500.14352/103585
UL https://hdl.handle.net/20.500.14352/103585
LA eng
NO Aranaz, I., Acosta, N., Revuelta, J., Bastida, A., Gómez-Casado, V., Civera, C., Garrido, L., García-Junceda, E., Heras, Á., Alcántara, A. R., Fernández-Mayoralas, A., & Doncel-Pérez, E. (2023). Fast and Sustained Axonal Growth by BDNF Released from Chitosan Microspheres. Marine drugs, 21(2), 91. https://doi.org/10.3390/md21020091
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Unión Europea
NO Fondo Europeo de Desarrollo Regional
DS Docta Complutense
RD 6 abr 2025