RT Journal Article
T1 Electrochemical immunosensor for rapid and sensitive determination of estradiol
A1 Ojeda Fernández, Irene
A1 Lopez-Montero, Judith
A1 Moreno Guzmán, María
A1 Janegitz, Bruno
A1 González Cortés, Araceli
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB This work describes the preparation of an electrochemical immunosensor for estradiol based on the surface modification of a screen printed carbon electrode with grafted p-aminobenzoic acid followed by covalent binding of streptavidin (Strept) and immobilization of biotinylated anti-estradiol (antiestradiol-Biotin). The hormone determination was performed by applying a competitive immunoassay with peroxidase-labelled estradiol (HRP–estradiol) and measurement of the amperometric response at −200 mV using hydroquinone (HQ) as redox mediator. The calibration curve for estradiol exhibited a linear range between 1 and 250 pg mL−1 (r = 0.990) and a detection limit of 0.77 pg mL−1 was achieved. Cross-reactivity studies with other hormones related with estradiol at physiological concentration levels revealed the practical specificity of the developed method for estradiol. A good reproducibility, with RSD = 5.9% (n = 8) was also observed. The operating stability of a single bioelectrode modified with anti-estradiol-Biotin-Strept was nine days when it was stored at 8 ◦C under humid conditions between measurements. The developed immunosensor was applied to the analysis of certified serum and spiked urine samples with good results
PB Elsevier
SN 0003-2670
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/91916
UL https://hdl.handle.net/20.500.14352/91916
LA eng
NO Ojeda, I., et al. «Electrochemical Immunosensor for Rapid and Sensitive Determination of Estradiol». Analytica Chimica Acta, vol. 743, septiembre de 2012, pp. 117-24. https://doi.org/10.1016/j.aca.2012.07.002.
NO Ministerio de Ciencia e Innovación (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 9 abr 2025