RT Journal Article
T1 Microsatellite typing of Lachancea thermotolerans for wine fermentation monitoring
A1 Vicente, Javier
A1 Navascués, Eva
A1 Benito, Santiago
A1 Marquina Díaz, Domingo
A1 Santos de la Sen, Antonio
AB Climate change is causing a lack of acidity during winemaking and oenologists use several solutions to cope with such a problem. Lachancea thermotolerans, which has the potential to tolerate the harsh physicochemical conditions of wine, has emerged as a promising alternative for pH management during winemaking and, currently, it is the most valuable yeast used for acidity control in wine. In this work a manageable method for L. thermotolerans genotyping based on a multiplexed microsatellite amplification in 6 different loci was developed. The proposed method was used to distinguish between 103 collection strains obtained from different geographical and isolation sources, and then challenged against a 429 L. thermotolerans isolates from several wineries and harvests. The procedure was also tested for fermentation monitoring and strain implantation. This approach was conceived to simplify the methodology available for L. thermotolerans genotyping, making it easy for applying in wine-related laboratories. This method can be applied to distinguish between L. thermotolerans strains in selection programs and to follow implantation of inoculated strains during winemaking with optimal results.
PB Elsevier
SN 0168-1605
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/88515
UL https://hdl.handle.net/20.500.14352/88515
LA eng
NO Vicente, Javier, et al. «Microsatellite Typing of Lachancea Thermotolerans for Wine Fermentation Monitoring». International Journal of Food Microbiology, vol. 394, junio de 2023, p. 110186. https://doi.org/10.1016/j.ijfoodmicro.2023.110186.
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 17 abr 2025