RT Journal Article
T1 Enzyme-induced modulation of the emission of upconverting nanoparticles: towards a new sensing scheme for glucose
A1 Del Barrio Redondo, Melisa
A1 Marcos, Susana de
A1 Cebolla, Vicente
A1 Heiland, Josef
A1 Wilhelm, Stefan
A1 Hirsch, Thomas
A1 Galbán, Javier
AB A new approach for the design of a fluorometric biosensor for continuous monitoring of glucose levels in biological samples based on near-infrared (NIR) excitation is described. The sensor combines the fluorescence of the enzyme glucose oxidase (GOx) chemically modified with a fluorescein derivative (FS) and the luminescent properties of upconverting luminescent nanoparticles (UCLNPs). Both, the chemically modified enzyme (GOx-FS) and the UCLNPs are immobilized in a poly(acrylamide) film as a physical support. The excitation of the UCLNPs with NIR light is of major advantage, since fluorescence background from the matrix is minimized. The upconverted luminescence is used to excite GOx-FS, which undergoes a change in the fluorescence intensity during the enzymatic reaction with glucose. The sensor comprises sufficient stability and covers the physiological range of glucose levels in blood. Furthermore, in a proof of principle experiment, the sensor system responds linearly to glucose concentrations in the range from 3.3 to 16.6. mM in flow injection analysis mode.
PB Elsevier
SN 0956-5663
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/93057
UL https://hdl.handle.net/20.500.14352/93057
LA eng
NO Del Barrio, Melisa, et al. «Enzyme-Induced Modulation of the Emission of Upconverting Nanoparticles: Towards a New Sensing Scheme for Glucose». Biosensors and Bioelectronics, vol. 59, septiembre de 2014, pp. 14-20.  https://doi.org/10.1016/j.bios.2014.02.076.
NO Ministerio de Economía y Competitividad (España)
NO Gobierno de Aragón
NO Consejo Superior de Investigaciones Científicas
DS Docta Complutense
RD 9 jun 2025