RT Journal Article
T1 Bovine peripheral blood MSCs chemotax towards inflammation and embryo implantation stimuli
A1 Calle, Alexandra
A1 Gutiérrez-Reinoso, Miguel Ángel
A1 Re, Michela Tatiana
A1 Blanco Murcia, Francisco Javier
A1 de la Fuente, Julio
A1 Monguió-Tortajada, Marta
A1 Enric Borràs, Francesc
A1 Yáñez-Mó, María
A1 Ramírez, Miguel Ángel
AB Mesenchymal stem cells (MSCs) have a great potential in regenerative medicine because of their multipotential and immunoregulatory capacities, while in early pregnancy they could participate in the immunotolerance of the mother towards the embryo. Peripheral blood constitutes an accessible source of MSCs. We successfully isolated peripheral blood MSC (pbMSCs) lines, with or without previous bone marrow mobilization. All pbMSCs lines obtained in both conditions presented classical MSC markers and properties, alkaline phosphatase activity and multipotent capacity to differentiate among adipogenic, osteogenic or chondrogenic lineages, and suppressed the proliferation of T cells. pbMSCs showed migratory capacity without cytokine stimulation while increasing their migration rate in the presence of inflammatory or embryo implantation stimuli. Interestingly, in contrast to MSCs derived from endometrial tissue, three pbMSCs lines also showed increased migration towards the IFN‐τ implantation cytokine. Moreover, the secretome produced by an early implantation stage embryonic trophectoderm cell line showed a chemoattractant effect in pbMSCs. Our results suggest that circulating MSCs are present in the peripheral blood under healthy conditions. The fact that both the inflammation and implantation signals induced pbMSCs chemotaxis highlights MSC heterogeneity and suggests that their migratory capacity may differ according to their tissue of origin and would suggest the possible active recruitment of MSCsfrom bone marrow during pregnancy to repress the immune response to prevent the embryo rejection by the maternal organism.
PB Wiley
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/116630
UL https://hdl.handle.net/20.500.14352/116630
LA eng
NO Calle, Alexandra, et al. «Bovine Peripheral Blood MSCs Chemotax towards Inflammation and Embryo Implantation Stimuli». Journal of Cellular Physiology, vol. 236, n.o 2, febrero de 2021, pp. 1054-67. https://doi.org/10.1002/jcp.29915.
NO Ministerio de Industria y Competitividad (España)
NO Ministerio de Ciencia e Innovación (España)
NO Ministerio de Transición Ecológica (España)
NO Fundación Biodiversidad
DS Docta Complutense
RD 10 abr 2025