RT Journal Article T1 SARS-CoV-2 Seroprevalence Studies in Pets, Spain A1 Sánchez Morales, Lidia A1 Barroso Arévalo, Sandra A1 Barasona García-Arévalo, José Ángel A1 Domínguez Rodríguez, Lucas José A1 Sánchez-Vizcaíno Rodríguez, José Manuel AB SARS-CoV-2 can infect domestic animals such as cats and dogs. The zoonotic origin of the disease requires surveillance on animals. Seroprevalence studies are useful tools for detecting previous exposure because the short period of virus shedding in animals makes detection of the virus difficult. We report on an extensive serosurvey on pets in Spain that covered 23 months. We included animals with exposure to SARS-CoV-2-infected persons, random animals, and stray animals in the study. We also evaluated epidemiologic variables such as human accumulated incidence and spatial location. We detected neutralizing antibodies in 3.59% of animals and showed a correlation between COVID-19 incidence in humans and positivity to antibody detection in pets. This study shows that more pets were infected with SARS-CoV-2 than in previous reports based on molecular research, and the findings highlight the need to establish preventive measures to avoid reverse zoonosis events. SN 1080-6040 SN 1080-6059 YR 2023 FD 2023-06 LK https://hdl.handle.net/20.500.14352/107523 UL https://hdl.handle.net/20.500.14352/107523 LA eng NO Barroso-Arévalo, S., Sánchez-Morales, L., Barasona, J. A., Domínguez, L., & Sánchez-Vizcaíno, J. M. (2023). SARS-CoV-2 Seroprevalence Studies in Pets, Spain. Emerging Infectious Diseases, 29(6), 1136-1142. https://doi.org/10.3201/eid2906.221737 NO Author contributions: conceptualization: S.B.-A., L.D., J.M.S.-V.; data curation: S.B.-A., L.S.-M., J.A.B.; formal analysis: S.B.-A., L.S.-M., J.A.B.; funding acquisition: J.M.S.-V., L.D.; laboratory analyses: S.B.-A., L.S.-M.; methodology: S.B.-A., L.S.-M., J.A.B., J.M.S.-V.; project administration: J.M.S.-V.; resources: L.D., J.M.S.-V.; software: S.B.-A., L.S.-M., J.A.B.; supervision: L.D., J.M.S.-V.; validation: S.B.-A., L.S.-M., J.A.B., J.M.S.-V.; visualization: S.B.-A., L.S.-M., J.A.B.; original draft preparation: S.B.-A., L.S.-M., J.A.B.; manuscript review and editing: S.B.-A., L.S.-M., J.A.B., L.D., J.M.S.-V. NO Instituto de Salud Carlos III NO European Union NO Comunidad de Madrid DS Docta Complutense RD 22 abr 2025