RT Journal Article
T1 Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized double–walled carbon nanotubes
A1 Sánchez Tirado, Esther
A1 Salvo, Coral
A1 González Cortés, Araceli
A1 Yáñez-Sedeño Orive, Paloma
A1 Langa, Fernando
A1 Pingarrón Carrazón, José Manuel
AB Dual screen-printed carbon electrodes modified with 4-carboxyphenyl-functionalized double-walled carbon nanotubes (HOOC-Phe-DWCNTs/SPCEs) have been used as scaffolds for the preparation of electrochemical immunosensors for the simultaneous determination of the cytokines Interleukin-1b (IL-1b) and factor necrosis tumor a (TNF-a). IL-1b. Capture antibodies were immobilized onto HOOC-Phe DWCNTs/SPCEs in an oriented form making using the commercial polymeric coating Mix&Go™. Sandwich type immunoassays with amperometric signal amplification through the use of poly-HRPstreptavidin conjugates and H2O2 as HRP substrate and hydroquinone as redox mediator were implemented. Upon optimization of the experimental variables affecting the immunosensor performance, the dual immunosensor allows ranges of linearity extending between 0.5 and 100 pg/mL and from 1 to 200 pg/mL for IL-1b and TNF-a, respectively, these ranges being adequate for the determination of the cytokines in clinical samples. The achieved limits of detection were 0.38 pg/mL (IL-1b) and 0.85 pg/mL (TNF-a). In addition, the dual immunosensor exhibits excellent reproducibility of the measurements, storage stability of the anti-IL-Phe-DWCNTs/SPCE and anti-TNF-Phe-DWCNTs/SPCE conjugates, and selectivity as well as negligible cross-talking. The dual immunosensor was applied to the simultaneous determination of IL-1b and TNF-a in human serum spiked at clinically relevant concentration levels and in real saliva samples.
PB Elsevier
SN 0003-2670
YR 2016
FD 2016-12-23
LK https://hdl.handle.net/20.500.14352/18045
UL https://hdl.handle.net/20.500.14352/18045
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid
DS Docta Complutense
RD 29 sept 2024