RT Journal Article
T1 Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters
A1 Gimeno Martos, Silvia
A1 Bosa, Luigia
A1 Lorenzo González, Pedro Luis
A1 Arias Álvarez, María
A1 Castellini, Cesare
A1 García Rebollar, Pilar
A1 García García, Rosa María
AB β-nerve growth factor (βNGF) plays a crucial role in reproductive physiology and sperm quality. Enzymatic activity of seminal plasma and vaginal fluids reduces available βNGF and it has been demonstrated that chitosan microspheres could protect rrβNGF from degradation. This study examined the effects of microencapsulated rrbNGF with chitosan on rabbit sperm viability, motility and capacitation status. Results showed that 0.5 and 1 μg/mL of microencapsulated rrβNGF, as well as free rrβNGF or empty microspheres, did not adversely affect sperm viability or total motility after 2 h of incubation. However, the highest progressivity kinetic parameters were observed with 1 μg/mL free rrβNGF, while the highest curvilinear velocity (VCL) occurred with 0.5 μg/mL microencapsulated rrβNGF. Empty chitosan microspheres did not induce acrosome reaction (AR), but both concentrations of free and rrβNGFch favoured AR during in vitro incubation. The study suggests that using chitosan spheres did not show any adverse effects on sperm traits, unlike free rβNGF and rrβNGFch promoted capacitation and AR. Further research is needed to explore the potential of rrβNGFch in modifying in vitro capacitation and inducing ovulation during artificial insemination.
PB Wiley
SN 0936-6768
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/109346
UL https://hdl.handle.net/20.500.14352/109346
LA eng
NO Gimeno-Martos, S., Bosa, L., Lorenzo, P. L., Arias-Alvarez, M., Castellini, C., García-Rebollar, P., & García-García, R. M. (2024). Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters. Reproduction in Domestic Animals, 59(Suppl. 3), e14636. https://doi.org/10.1111/rda.14636
NO Ministerio de Economía, Comercio y Empresa (España)
NO European Commission
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 19 mar 2026