RT Journal Article
T1 Selective digestive decontamination solution used as “lock therapy” prevents and eradicates bacterial biofilm in an in vitro bench-top model
A1 Pérez Granda, María Jesús
A1 Alonso, Beatriz
A1 Zavala, Ricardo
A1 Latorre, María Consuelo
A1 Hortal Iglesias, Francisco Javier
A1 Samaniego, Rafael
A1 Bouza Santiago, Emilio
A1 Muñoz García, Patricia Carmen
A1 Guembe, María
AB BackgroundMost preventing measures for reducing ventilator-associated pneumonia (VAP) are based mainly on the decolonization of the internal surface of the endotracheal tubes (ETTs). However, it has been demonstrated that bacterial biofilm can also be formed on the external surface of ETTs. Our objective was to test in vitro the efficacy of selective digestive decontamination solution (SDDs) onto ETT to prevent biofilm formation and eradicate preformed biofilms of three different microorganisms of VAP.MethodsWe used an in vitro model in which we applied, at the subglottic space of ETT, biofilms of either P. aeruginosa ATCC 15442, or E. coli ATCC 25922, or S. aureus ATCC 29213, and the SDDs at the same time (prophylaxis) or after 72 h of biofilm forming (treatment). ETT were incubated during 5 days with a regimen of 2 h-locks. ETT fragments were analyzed by sonication and confocal laser scanning microscopy to calculate the percentage reduction of cfu and viable cells, respectively.ResultsMedian (IQR) percentage reduction of live cells and cfu/ml counts after treatment were, respectively, 53.2% (39.4%—64.1%) and 100% (100%–100.0%) for P. aeruginosa, and 67.9% (46.7%–78.7%) and 100% (100%–100.0%) for E. coli. S. aureus presented a complete eradication by both methods. After prophylaxis, there were absence of live cells and cfu/ml counts for all microorganisms.ConclusionsSDDs used as “lock therapy” in the subglottic space is a promising prophylactic approach that could be used in combination with the oro-digestive decontamination procedure in the prevention of VAP.
PB BioMed Central Ltd
SN 1476-0711
YR 2020
FD 2020-09-23
LK https://hdl.handle.net/20.500.14352/120111
UL https://hdl.handle.net/20.500.14352/120111
LA eng
NO Pérez-Granda MJ, Alonso B, Zavala R, Latorre MC, Hortal J, Samaniego R, et al. Selective digestive decontamination solution used as «lock therapy» prevents and eradicates bacterial biofilm in an in vitro bench-top model. Annals of clinical microbiology and antimicrobials. 2020;19(1):44.
NO M. Guembe cuenta con el apoyo del Programa Miguel Servet (ISCIII-MICINN, CP13/00268) del Fondo de Investigación en Salud (FIS) del Instituto de Salud Carlos III (ISCIII), Madrid, España. Beatriz Alonso recibe apoyo de la Consejería de Educación, Juventud y Deporte de la Comunidad de Madrid y del Fondo Social Europeo (PEJ15/BIO/AI-0406). El estudio fue financiado parcialmente mediante subvenciones del ISCIII (PI18/00045) y del Fondo Europeo de Desarrollo Regional (FEDER) “Una manera de hacer Europa”, así como por ayudas del Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM).
DS Docta Complutense
RD 23 ene 2026