RT Journal Article
T1 Cux‐1 and Cux‐2 control the development of Reelin expressing cortical interneurons
A1 Cubelos, Beatriz
A1 Kim, Seonhee
A1 Redondo, Juan Miguel
A1 Walsh, Christopher
A1 Nieto, Marta
A1 Sebastián Serrano, Álvaro
AB Homeodomain transcription factors play important roles in the specification and differentiation of neuronal subpopulations. In the cerebral cortex, the expression patterns of Cux‐1 and Cux‐2 in the medial ganglionic eminence (MGE) suggest a role for these transcription factors in the development of interneurons, a heterogeneous neuronal population. In this report, we describe expression of Cux‐1 and Cux‐2 proteins in Reelin‐secreting interneurons of the cortical plate, but not in calretinin or parvalbumin subpopulations. The role of Cux genes in the development of Reelin positive neurons was studied using Cux‐1 and Cux‐2 knockout mice. These experiments demonstrate that Cux‐1−/−; Cux‐2−/− double mutation is embryonically lethal. Although this phenotype is highly penetrant, a small proportion of mice develop to birth (P0). Analysis of these animals demonstrate that expression of Reelin is completely absent in layers II–IV of Cux‐1−/−; Cux‐2−/− double mutant mice, but it is not affected in the cortex of Cux‐1−/− or Cux‐2−/− single mutants. No Cux‐1−/−; Cux‐2−/− double‐mutant were collected after P0. Since, GABA‐ergic populations mature at late postnatal stages, this did not allow us to analyze the expression of subclass specific markers and define the affected interneuron subpopulations. Our analysis of Cux‐1−/−; Cux‐2−/− double mutant thus demonstrates essential yet redundant roles for Cux‐1 and Cux‐2 in specifying Reelin expressing cortical interneurons. © 2008 Wiley Periodicals, Inc. Develop Neurobiol, 2008.
PB Wiley
SN 1932-8451
SN 1932-846X
YR 2008
FD 2008
LK https://hdl.handle.net/20.500.14352/96241
UL https://hdl.handle.net/20.500.14352/96241
LA eng
NO Cubelos B, Sebastián-Serrano A, Kim S, Redondo JM, Walsh C, Nieto M. Cux-1 and Cux-2 control the development of Reelin expressing cortical interneurons. Dev Neurobiol. 2008 Jun;68(7):917-25. doi: 10.1002/dneu.20626. PMID: 18327765; PMCID: PMC2938960.
DS Docta Complutense
RD 10 abr 2025