RT Journal Article
T1 A viscometric study of the biodegradation of photographic gelatin by fungi isolated from cinematographic films
A1 Abrusci, Concepción
A1 Marquina Díaz, Domingo
A1 Del Amo, Alfonso
A1 Corrales, Teresa
A1 Catalina, Fernando
AB The biodegradation of photographic gelatin grade (Bloom 225) material was studied by viscometry in aqueous solution (at 37 °C, 6.67% w/w) using filamentous fungi isolated and identified from cinematographic film stored in different Spanish archives. From viscosity data, different variables such as molecular weight and chain scission were calculated. To ensure initial spore suspension concentration was standardized for all the biodegradation experiments, a correlation between transmittance at 530 nm of fungal spore suspensions and the corresponding cytometric determination of populations was established for all the fungal strains studied in this work. The bioassay experiments were carried out at 25 and 4 °C using an initial concentration of fungi of 4.5×105 conidia/mL except in the case of the genus Alternaria, where the concentration was 10 times lower. The fungal strains were three species of Aspergillus, i.e., A .ustus, A. nidulans var. nidulans, A. versicolor, seven Penicillium chrysogenum strains, and Cladosporium cladosporioides, Alternaria alternata, Mucor racemosus, Phoma glomerata, and Trichoderma longibrachiatum. All were gelatinase positive. Through the viscosity decay profiles with bioassay-time and the corresponding calculated chain scission, the relative quantitative gelatinase efficiency of these fungi has been evaluated.
PB Elsevier
SN 0964-8305
YR 2006
FD 2006-09-20
LK https://hdl.handle.net/20.500.14352/106881
UL https://hdl.handle.net/20.500.14352/106881
LA eng
NO Abrusci, C., D. Marquina, A. Santos, et al. «A Chemiluminescence Study on Degradation of Gelatine». Journal of Photochemistry and Photobiology A: Chemistry, vol. 185, n.o 2-3, enero de 2007, pp. 188-97. DOI.org (Crossref), https://doi.org/10.1016/j.jphotochem.2006.06.003.
NO Ministerio de Educación y Ciencia (España)
NO Filmoteca Española
DS Docta Complutense
RD 2 oct 2024