RT Journal Article
T1 Unravelling the orientation of the inositol-biphosphate ring and its dependence on phosphatidylinositol 4,5-bisphosphate cluster formation in model membranes
A1 Santamaria, Andreas
A1 Javier Carrascosa-Tejedor, 
A1 Guzmán Solís, Eduardo
A1 Zaccai, Nathan R.
A1 Maestro, Armando
AB HypothesisInositol phospholipids are well known to form clusters in the cytoplasmic leaflet of the plasma membrane that are responsible for the interaction and recruitment of proteins involved in key biological processes like endocytosis, ion channel activation and secondary messenger production. Although their phosphorylated inositol ring headgroup plays an important role in protein binding, its orientation with respect to the plane of the membrane and its lateral packing density has not been previously described experimentally.ExperimentsHere, we study phosphatidylinositol 4,5-bisphosphate (PIP2) planar model membranes in the form of Langmuir monolayers by surface pressure-area isotherms, Brewster angle microscopy and neutron reflectometry to elucidate the relation between lateral (in-plane) and perpendicular (out-of-plane) molecular organization of PIP2.FindingsDifferent surface areas were explored through monolayer compression, allowing us to correlate the formation of transient PIP2 clusters with the change in orientation of the inositol-biphosphate headgroup, which was experimentally determined by neutron reflectometry.
PB Elsevier
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/95202
UL https://hdl.handle.net/20.500.14352/95202
LA eng
NO Santamaria, Andreas, et al. «Unravelling the Orientation of the Inositol-Biphosphate Ring and Its Dependence on Phosphatidylinositol 4,5-Bisphosphate Cluster Formation in Model Membranes». Journal of Colloid and Interface Science, vol. 629, enero de 2023, pp. 785-95. https://doi.org/10.1016/j.jcis.2022.09.095.
NO Eusko Jaurlaritza
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 6 oct 2024