RT Book, Section
T1 Genome Editing of Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes to Model Genetic Ocular Diseases
A1 Getachew, Heran
A1 Chinchilla Rodríguez, Blanca
A1 Fernandez-Godino, Rosario
A2 Turksen, K
AB Genome editing with the use of CRISPR/Cas9 ribonucleoprotein complexes of induced pluripotent stem cells can be used to model many diseases. The combination of stem cells and gene editing technologies is a valuable tool to study ocular disorders, as many have been identified to be caused by specific genetic mutations. This protocol provides experimentally derived guidelines for genome editing of human induced pluripotent stem cells (iPSCs) using CRISPR/Cas9 ribonucleoprotein complexes to generate iPSCs harboring single nucleotide variants from ocular disorders. Edited iPSC can be further differentiated into retinal cells in order to study disease mechanisms as well as screen potential therapies.
PB Humana Press
SN 978-1-0716-2584-2
SN 1940-6029
YR 2021
FD 2021-06-15
LK https://hdl.handle.net/20.500.14352/116689
UL https://hdl.handle.net/20.500.14352/116689
LA eng
NO Getachew, H., Chinchilla, B., Fernandez-Godino, R. (2021). Genome Editing of Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes to Model Genetic Ocular Diseases. In: Turksen, K. (eds) Induced Pluripotent Stem Cells and Human Disease. Methods in Molecular Biology, vol 2549. Humana, New York, NY. https://doi.org/10.1007/7651_2021_409
DS Docta Complutense
RD 21 ene 2026