RT Journal Article
T1 Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress
A1 Sobrino Plata, Juan
A1 Meyssen, Dirk
A1 Cuypers, Ann
A1 Escobar, Carolina
A1 Hernández, Luis
AB Background. Glutathione (GSH) plays a dual role under heavy metal stress, as antioxidant metabolite and as precursor of phytochelatins (PCs). Studying the responses of the GSH metabolism to heavy metals is important to improve tolerance.Methods. We studied the oxidative stress signature of three γ-glutamylcysteine synthetase (γECS) Arabidopsis thaliana allele mutants (rax1-1, cad2-1, and pad2-1), first enzymatic step in the GSH synthetic pathway, when treated with 10 μM Cd or Hg for 72 h.Results. GSH concentration was lower in the mutants (45 % rax1-1; 30 % cad2-1; and 20 % pad2-1), which was also associated with inferior translocation of Cd or Hg to shoots, than in wild type Col-0. Glutathione reductase (GR) and NADPH-oxidase activities were inhibited in roots, phytotoxic effects consistently more pronounced in the mutants, particularly in pad2-1. Non-photochemical quenching augmented with exposure time to Cd or Hg in Col-0, but not so in the γECS mutants. Mercury caused severe damage in cad2-1 and pad2-1 root proteins profile; toxic effects confirmed by GR and H+-ATPase immunodetection. PCs appeared in Col-0 roots under metal stress, and surprisingly accumulated in rax1-1. γECS immunodetection revealed its overexpression in rax1-1.Conclusion. A minimum amount of GSH may be required for adequate metal tolerance, where γECS expression could compensate GSH deficiency under stress.
PB Springer
SN 0032-079X
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/99658
UL https://hdl.handle.net/20.500.14352/99658
LA eng
NO Sobrino-Plata, J., Meyssen, D., Cuypers, A. et al. Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil 377, 369–381 (2014). https://doi.org/10.1007/s11104-013-2006-4
NO This work was funded by the Ministry of Economy and Competitivity (PROBIOMET AGL2010-15151), Fundación Ramón Areces, and Junta Comunidades Castilla-La Mancha (FITOALMA2, POII10-0087-6458).
NO Ministerio de Economía y Competitividad (España)
NO Junta de Comunidades de Castilla-La Mancha
NO Fundación Ramón Areces
DS Docta Complutense
RD 13 abr 2025