%0 Journal Article
%A Serafín González-Carrato, Verónica
%A Arévalo Pérez, Beatriz
%A Martínez-García, Gonzalo
%A Aznar-Poveda, Juan
%A Lopez-Pastor, José Antonio
%A Beltrán-Sánchez, J. Francisco
%A Garcia-Sanchez Antonio, Javier
%A Garcia-Haro, Juan
%A Campuzano Ruiz, Susana
%A Yáñez-Sedeño Orive, Paloma
%A Pingarrón Carrazón, José Manuel
%T Enhanced determination of fertility hormones in saliva at disposable immunosensing platforms using a custom designed field-portable dual potentiostat
%D 2019
%@ 0925-4005
%U https://hdl.handle.net/20.500.14352/95389
%X This work describes a new electroanalytical device for the simultaneous and reliable determination of two fertility relevant hormones (luteinizing hormone, LH, and progesterone, P4) in saliva. The device is constructed using a custom designed and field-portable potentiostat where dual disposable immunosensing platform are connected. The immunosensors are based on sandwich-type and competitive immunoassays implemented onto magnetic microbeads (MBs) functionalized with Neutravidin and Protein G for the determination of LH and P4, respectively. Amperometric detection performed at −0.20 V vs the Ag pseudo-reference electrode using the H2O2/hydroquinone (HQ) system was employed as the transduction technique after placing the MBs with immunocomplexes for each target hormone on the appropriate working electrode of screen-printed dual carbon electrodes (SPdCEs). The method exhibits high sensitivity and selectivity for the target hormones providing detection limits of 1.7 pg mL−1 and 0.10 mIU mL−1 for P4 and LH, respectively, with a 1 h test time. The applicability of the method was confirmed by determining both hormones in saliva samples from different volunteers providing results comparable to those obtained using amperometric transduction with a commercial potentiostat and with ELISA methodologies involving the same immunoreagents for each target hormone.
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