RT Journal Article
T1 Enhanced determination of fertility hormones in saliva at disposable immunosensing platforms using a custom designed field-portable dual potentiostat
A1 Serafín González-Carrato, Verónica
A1 Arévalo Pérez, Beatriz
A1 Martínez-García, Gonzalo
A1 Aznar-Poveda, Juan
A1 Lopez-Pastor, José Antonio
A1 Beltrán-Sánchez, J. Francisco
A1 Garcia-Sanchez Antonio, Javier
A1 Garcia-Haro, Juan
A1 Campuzano Ruiz, Susana
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB This work describes a new electroanalytical device for the simultaneous and reliable determination of two fertility relevant hormones (luteinizing hormone, LH, and progesterone, P4) in saliva. The device is constructed using a custom designed and field-portable potentiostat where dual disposable immunosensing platform are connected. The immunosensors are based on sandwich-type and competitive immunoassays implemented onto magnetic microbeads (MBs) functionalized with Neutravidin and Protein G for the determination of LH and P4, respectively. Amperometric detection performed at −0.20 V vs the Ag pseudo-reference electrode using the H2O2/hydroquinone (HQ) system was employed as the transduction technique after placing the MBs with immunocomplexes for each target hormone on the appropriate working electrode of screen-printed dual carbon electrodes (SPdCEs). The method exhibits high sensitivity and selectivity for the target hormones providing detection limits of 1.7 pg mL−1 and 0.10 mIU mL−1 for P4 and LH, respectively, with a 1 h test time. The applicability of the method was confirmed by determining both hormones in saliva samples from different volunteers providing results comparable to those obtained using amperometric transduction with a commercial potentiostat and with ELISA methodologies involving the same immunoreagents for each target hormone.
PB Elsevier
SN 0925-4005
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/95389
UL https://hdl.handle.net/20.500.14352/95389
LA eng
NO Serafín V, Arévalo B, Martínez-García G,  Aznar-Poveda J, Lopez-Pastor J.A, Beltrán-Sánchez J.F, Garcia-Sanchez A.J, Garcia-Haro J, Campuzano S, Yáñez-Sedeño P, Pingarrón  J.M. Enhanced determination of fertility hormones in saliva at disposable immunosensing platforms using a custom designed field-portable dual potentiostat.  	Sens. Actuators, B. 2019 Agosto 7; 299: 126934
NO Ministerio de Economía y Competitividad (España)
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid
NO European Commission
NO Región de Murcia
DS Docta Complutense
RD 6 abr 2025