RT Journal Article
T1 Synergistic effect of Si-hydroxyapatite coating and VEGF adsorption on Ti6Al4V-ELI scaffolds for bone regeneration in an osteoporotic bone environment.
A1 Izquierdo Barba, Isabel
A1 Santos-Ruiz, L
A1 Becerra, J
A1 Feito Castellano, María José
A1 Fernandez-Villa, D
A1 Serrano, M.C
A1 Diaz-Gúemes, I
A1 Fernandez-Tome, B
A1 Enciso, S
A1 Sanchez-Margallo, F.M
A1 Monopoli, D
A1 Alfonso, H
A1 Portolés Pérez, María Teresa
A1 Arcos Navarrete, Daniel
A1 Vallet Regí, María Dulce Nombre
AB The osteogenic and angiogenic responses to metal macroporous scaffolds coated with silicon substituted hydroxyapatite (SiHA) and decorated with vascular endothelial growth factor (VEGF) have been evaluated in vitro and in vivo. Ti6Al4V-ELI scaffolds were prepared by electron beam melting and subsequently coated with Ca10(PO4)5.6(SiO4)0.4(OH)1.6 following a dip coating method. In vitro studies demonstrated that SiHA stimulates the proliferation of MC3T3-E1 pre-osteoblastic cells, whereas the adsorption of VEGF stimulates the proliferation of EC2 mature endothelial cells. In vivo studies were carried out in an osteoporotic sheep model, evidencing that only the simultaneous presence of both components led to a significant increase of new tissue formation in osteoporotic bone.STATEMENT OF SIGNIFICANCEReconstruction of bones after severe trauma or tumors extirpation is one of the most challenging tasks in the field of orthopedic surgery. This scenario is even more complicated in the case of osteoporotic patients, since their bone regeneration capability is decreased. In this work we present a porous implant that promotes bone regeneration even in osteoporotic bone. By coating the implant with an osteogenic bioceramics such as silicon substituted hydroxyapatite and subsequent adsorption of vascular endothelial growth factor, these implants stimulate the bone ingrowth when they are implanted in osteoporotic sheep.
PB ELSEVIER SCIENCE BV
SN 1742-7061
YR 2018
FD 2018-11-13
LK https://hdl.handle.net/20.500.14352/12928
UL https://hdl.handle.net/20.500.14352/12928
LA eng
NO RESEARCHER ID M-9921-2014 (Isabel Izquierdo Barba)ORCID 0000-0002-4139-4646 (Isabel Izquierdo Barba)RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez)ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete)ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)
NO Unión Europea. H2020
NO Ministerio de Economía y Competitividad (MINECO)
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 18 abr 2025