RT Journal Article
T1 Acid-sensing ion channels (ASICs) 2 and 4.2 are expressed in the retina of the adult zebrafish
A1 Viña Fernández, Eliseo
A1 Parisi, Valentina
A1 Sánchez Ramos, Celia
A1 Cabo, Roberto
A1 Guerrera, María Cristina
A1 Quirós, Luis Manuel
A1 Germaná, Antonio M.
A1 Vega, José Antonio
A1 García Suárez, Olivia
AB Acid-sensing ion channels (ASICs) are H+-gated, voltage-insensitive cation channels involved in synaptic transmission, mechanosensation and nociception. Different ASICs have been detected in the retina of mammals but it is not known whether they are expressed in adult zebrafish, a commonly used animal model to study the retina in both normal and pathological conditions. We study the expression and distribution of ASIC2 and ASIC4 in the retina of adult zebrafish and its regulation by light using PCR, in situ hybridization, western blot and immunohistochemistry. We detected mRNA encoding zASIC2 and zASIC4.2 but not zASIC4.1. ASIC2, at the mRNA or protein level, was detected in the outer nuclear layer, the outer plexiform layer, the inner plexiform layer, the retinal ganglion cell layer and the optic nerve. ASIC4 was expressed in the photoreceptors layer and to a lesser extent in the retinal ganglion cell layer. Furthermore, the expression of both ASIC2 and ASIC4.2 was down-regulated by light and darkness. These results are the first demonstration that ASIC2 and ASIC4 are expressed in the adult zebrafish retina and suggest that zebrafish could be used as a model organism for studying retinal pathologies involving ASICs.
PB Springer Verlag
SN 0302-766X
YR 2015
FD 2015-05-01
LK https://hdl.handle.net/20.500.14352/23079
UL https://hdl.handle.net/20.500.14352/23079
LA eng
NO The online version of this article contains supplementary material, which is available to authorized users.Received: 11 March 2014 /Accepted: 27 November 2014 /Published online: 15 January 2015
DS Docta Complutense
RD 5 abr 2025