RT Journal Article
T1 1,3-Propanediol production from glycerol with a novel biocatalyst Shimwelliablattae ATCC 33430: Operational conditions and kinetics in batch cultivations
A1 Rodríguez, Alberto
A1 Wojtusik, Mateusz
A1 Ripoll, Vanessa
A1 Santos, Victoria E.
A1 García-Ochoa, F.
AB Shimwellia blattae ATCC 33430 as biocatalyst in the conversion of 1,3-propanediol from glycerol is herein evaluated. Several operational conditions in batch cultivations, employing pure and raw glycerol as sole carbon source, were studied. Temperature was studied at shaken bottle scale, while pH control strategy, together with the influence of raw glycerol and its impurities during fermentation were studied employing a 2 L STBR. Thereafter, fluid dynamic conditions were considered by changing the stirring speed and the gas supply (air or nitrogen) in the same scale-up experiments. The best results were obtained at a termperature of 37ºC, an agitation rate of 200 rpm, with free pH evolution from 6.9 and subsequent control at 6.5 and no gas supply during the fermentation, employing an initial concentration of 30 g/L of raw glycerol. Under these conditions, the biocatalyst is competitive, leading to results in line with other previous works in the literature in batch conditions, reaching a final concentration of 1,3-propanediol of 13.84 g/L, with a yield of 0.45 g/g and a productivity of 1.19 g/(L·h) fromraw glycerol.
PB Elsevier
SN 0960-8524(Print) ; 1873-2976 (on line)
YR 2015
FD 2015-10-23
LK https://hdl.handle.net/20.500.14352/24235
UL https://hdl.handle.net/20.500.14352/24235
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO BLICEL
NO POAP
NO VALOGLI
DS Docta Complutense
RD 2 may 2024