RT Journal Article
T1 Maternal Diet Determines Milk Microbiome Composition and Offspring Gut Colonization in Wistar Rats
A1 Martínez-Oca, Paula
A1 Alba Rubio, Claudio
A1 Sánchez Roncero, Alicia
A1 Fernández-Marcelo, Tamara
A1 Martín, María Ángeles
A1 Escrivá Pons, Fernando
A1 Rodríguez Gómez, Juan Miguel
A1 Álvarez Escolá, Carmen
A1 Fernández Millán, Elisa
AB Mother’s milk contains a unique microbiome that plays a relevant role in offspring health. We hypothesize that maternal malnutrition during lactation might impact the microbial composition of milk and affect adequate offspring gut colonization, increasing the risk for later onset diseases. Then, Wistar rats were fed ad libitum (Control, C) food restriction (Undernourished, U) during gestation and lactation. After birth, offspring feces and milk stomach content were collected at lactating day (L)4, L14 and L18. The V3–V4 region of the bacterial 16S rRNA gene was sequenced to characterize bacterial communities. An analysis of beta diversity revealed significant disparities in microbial composition between groups of diet at L4 and L18 in both milk, and fecal samples. In total, 24 phyla were identified in milk and 18 were identified in feces, with Firmicutes, Proteobacteria, Actinobacteroidota and Bacteroidota collectively representing 96.1% and 97.4% of those identified, respectively. A higher abundance of Pasteurellaceae and Porphyromonas at L4, and of Gemella and Enterococcus at L18 were registered in milk samples from the U group. Lactobacillus was also significantly more abundant in fecal samples of the U group at L4. These microbial changes compromised the number and variety of milk–feces or feces–feces bacterial correlations. Moreover, increased offspring gut permeability and an altered expression of goblet cell markers TFF3 and KLF3 were observed in U pups. Our results suggest that altered microbial communication between mother and offspring through breastfeeding may explain, in part, the detrimental consequences of maternal malnutrition on offspring programming.
PB MDPI
SN 2072-6643
YR 2023
FD 2023-10-10
LK https://hdl.handle.net/20.500.14352/103652
UL https://hdl.handle.net/20.500.14352/103652
LA eng
NO Martínez-Oca, P., Alba, C., Sánchez-Roncero, A., Fernández-Marcelo, T., Martín, M. Á., Escrivá, F., Rodríguez, J. M., Álvarez, C., & Fernández-Millán, E. (2023). Maternal Diet Determines Milk Microbiome Composition and Offspring Gut Colonization in Wistar Rats. Nutrients, 15(20), 4322. https://doi.org/10.3390/nu15204322
NO Consorcio Centro de Investigación Biomédica en Red (España)
NO Instituto de Salud Carlos III (España)
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 11 abr 2025