RT Journal Article
T1 Exercise and tumor proteome: insights from a neuroblastoma model
A1 González Gálvez, Beatriz
A1 Martín Ruiz Valdepeñas, María Asunción
A1 Plaza-Florido, Abel
A1 López, Juan A.
A1 Santos-Lozano, Alejandro
A1 Zazo, Sandra
A1 Rincón-Castanedo, Cecilia
A1 Lumbreras, Jorge
A1 Terrón-Camero, Laura C.
A1 López-Soto, Alejandro
A1 Andrés-León, Eduardo
A1 González-Murillo, África
A1 Rojo, Federico
A1 Ramírez, Manuel
A1 Lucía, Alejandro
A1 Fluza-Luces, Carmen
AB The impact of exercise on pediatric tumor biology is essentially unknown. We  explored the effects of regular exercise on tumor proteome profile (as assessed  with liquid chromatography with tandem mass spectrometry) in a mouse model of  one of the most aggressive childhood malignancies, high-risk neuroblastoma  (HR-NB). Tumor samples of 14 male mice (aged 6-8 wk) that were randomly  allocated into an exercise (5-wk combined aerobic and resistance training) or  nonexercise control group (6 and 8 mice/group, respectively) were analyzed. The  Search Tool for the Retrieval of Interacting Genes/Proteins database was used to  generate a protein-protein interaction (PPI) network and enrichment analyses.  The Systems Biology Triangle (SBT) algorithm was applied for analyses at the  functional category level. Tumors of exercised mice showed a higher and lower  abundance of 101 and 150 proteins, respectively, than controls [false discovery  rate (FDR) < 0.05]. These proteins were enriched in metabolic pathways, amino  acid metabolism, regulation of hormone levels, and peroxisome  proliferator-activated receptor signaling (FDR < 0.05). The SBT algorithm  indicated that 184 and 126 categories showed a lower and higher abundance,  respectively, in the tumors of exercised mice (FDR < 0.01). Categories with  lower abundance were involved in energy production, whereas those with higher  abundance were related to transcription/translation, apoptosis, and tumor  suppression. Regular exercise altered the abundance of hundreds of intratumoral  proteins and molecular pathways, particularly those involved in energy  metabolism, apoptosis, and tumor suppression. These findings provide preliminary  evidence of the molecular mechanisms underlying the potential effects of  exercise in HR-NB. NEW & NOTEWORTHY We used liquid chromatography with tandem  mass spectrometry to explore the impact of a 5-wk exercise intervention on the  tumor proteome profile in a mouse model of one of the most aggressive childhood  malignancies, high-risk neuroblastoma. Exercise altered the abundance of  hundreds of proteins and pathways, particularly those involved in energy  metabolism and tumor suppression. These molecular changes could mediate, at  least partly, the potential antitumorigenic effects of exercise.
PB American Physiological Society
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/121473
UL https://hdl.handle.net/20.500.14352/121473
LA eng
NO Plaza-Florido A, Gálvez BG, López JA, Santos-Lozano A, Zazo S, Rincón-Castanedo C, et al. Exercise and tumor proteome: insights from a neuroblastoma model. Physiological Genomics 2024;56:833–44. https://doi.org/10.1152/physiolgenomics.00064.2024.
NO A.P.-F. is supported in part by National Institutes of Health Grant U01TR002004 (REACH project). Research by A.L. and C.F.- L. is funded by the Wereld Kanker Onderzoek Fonds (WKOF) as part of the World Cancer Research Fund International grant program (Grant IIG_FULL_2021_007) and the Spanish Ministry of Science and Innovation [Fondo de Investigaciones Sanitarias (FIS)] and Fondos FEDER (Grant PI18/00139). Research by C.F.-L. is funded by the Spanish Ministry of Science and Innovation [Fondo de Investigaciones Sanitarias (FIS)] and Fondos FEDER (Grants PI20/00645, PI23/00396, and FORT23/00023), by the Ministerio de Ciencia e Innovacion (Grant CNS2023-144144), and   by a Miguel Servet postdoctoral contract granted by Instituto de Salud Carlos III (CP18/00034). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia, Innovacion Y Universidades (MICIU) and the Pro CNIC Foundation,   and the Severo Ochoa Center of Excellence (Grant CEX2020- 001041-S funded by MICIU/AEI/10.13039/501100011033)
NO National Institutes of Health
NO Wereld Kanker Onderzoek Fonds (WKOF)
NO Ministerio de Ciencia e Innovación (España)
NO European Commission
NO Instituto de Salud Carlos III
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Fundación Pro CNIC
DS Docta Complutense
RD 22 ene 2026