%0 Journal Article
%A Pérez Luz, Sara
%A Yañez, María Adela
%A Catalan, Vicente
%T Identification of waterborne bacteria by the analysis of 16S–23S rRNA intergenic spacer region
%D 2004
%U https://hdl.handle.net/20.500.14352/94437
%X Aim: In this study, we evaluated, the use of universal primers, specific for the 16S-23S rRNA intergenic region, to detect and identify nine species that are of high interest for the microbiological control of water.Methods and results: The analysis of the fragments was carried out using a High Resolution acrylamide/bisacrylamide gels in a fluorescent automated DNA sequencer. The results showed specific profiles for each of the nine species but this technique failed to detect simultaneously micro-organisms in samples containing a mixed population.Conclusion: Nevertheless, the electrophoretic profiles obtained provided a very useful tool for the rapid and specific identification of water isolates.Significance and impact of the study: A possible new methodology for a rapid identification of pathogens in water.
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