RT Journal Article
T1 Additives enhancing enzymatic hydrolysis of lignocellulosic biomass
A1 Rocha Martín, Javier
A1 Martínez-Bernal, Claudio
A1 Pérez-Cobas, Yolanda
A1 Reyes-Sosa, Francisco Manuel
A1 Díez García, Bruno
AB Linked to the development of cellulolytic enzyme cocktails from Myceliophthora thermophila, we studied the effect of different additives on the enzymatic hydrolysis yield. The hydrolysis of pretreated corn stover (PCS), sugar cane straw (PSCS) and microcrystalline cellulose (Avicel) was performed under industrial conditions using high solid loadings, limited mixing, and low enzyme dosages. The addition of polyethylene glycol (PEG4000) allowed to increase the glucose yields by 10%, 7.5%, and 32%, respectively in the three materials. PEG4000 did not have significant effect on the stability of the main individual enzymes but increased beta-glucosidase and endoglucanase activity by 20% and 60% respectively. Moreover, the presence of PEG4000 accelerated cellulase-catalyzed hydrolysis reducing up to 25% the liquefaction time. However, a preliminary economical assessment concludes that even with these improvements, a lower contribution of PEG4000 to the 2G bioethanol production costs would be needed to reach commercial feasibility.
PB Elsevier
SN 0960-8524
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/94103
UL https://hdl.handle.net/20.500.14352/94103
LA eng
NO Rocha-Martín, Javier, et al. «Additives Enhancing Enzymatic Hydrolysis of Lignocellulosic Biomass». Bioresource Technology, vol. 244, noviembre de 2017, pp. 48-56. https://doi.org/10.1016/j.biortech.2017.06.132
DS Docta Complutense
RD 11 abr 2025